New Anesthetic Regimen on Canine Model of Septic Shock

犬类基因微阵列技术最近已成为可能。这项技术使用血液样本来检测在疾病过程中表达的基因。这项研究将调查基因表达模式的差异是否与脓毒症期间的疾病严重程度相关，并区分幸存者和非幸存者。 这项研究旨在研究脓毒症的遗传机制。通过使用四种不同的细菌水平(0、6、12、18 x 109 cfu)，我们将产生一系列严重的感染，这应该会产生不同的基因表达模式。这种设计允许检测在产生败血症病理生理过程中所涉及的遗传通路的激活和失活的趋势。此外，与小动物模型不同，犬类败血症模型允许随着时间的推移进行连续测量，并允许比较幸存者和非幸存者的基因变化。这些信息可以确定新的治疗靶点，新的测试将允许确定脓毒症的存在和严重程度，并可能确定预测生存的标志。

Canine gene microarray technology has recently become available. This technology uses a blood sample to detect genes that are being expressed during the course of an illness. This study will investigate whether differences in patterns of gene expression correlate with the severity of illness during sepsis and differentiate between survivors and non-survivors. The study is designed to examine the genetic mechanisms of sepsis. By using four different bacterial levels (0, 6, 12, 18 x 109 CFUs), we will produce a range of severity of infection which should produce distinct patterns of gene expression. This design allows for the detection of trends in both activation and inactivation of the genetic pathways involved in producing septic pathophysiology. In addition, unlike small animal models, the canine sepsis model allows for serial measurements over time and will allow for comparison of genetic changes in survivors vs. non-survivors. This information could identify new targets for therapy, new tests that will allow for the determination of the presence and severity of sepsis, and possibly markers that predict survival