Post-transcriptional control of gene expression

基因表达的转录后控制

• 批准号: 6761390
• 负责人: ROY PARKER
• 金额: $ 0.3万
• 依托单位: FEDERATION OF AMER SOC FOR EXPER BIOLOGY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-15 至 2005-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6761390
• 关键词: gene expression; meeting /conference /symposium; posttranscriptional RNA processing; travel

DESCRIPTION (provided by applicant): We are requesting partial support for a FASEB Conference on "post-transcriptional regulation of gene expression: mechanisms of mRNA decay" to be held in Tucson AZ on June 26-July 1, 2004. Turnover of mRNA is a key, but frequently unrecognized, regulator of gene expression as the stability of an mRNA dictates its ultimate steady-state level. Moreover, programmed instability is an important mechanism of repressing gene expression. In addition, it is increasingly recognized that most organisms employ means of surveillance of mRNA quality to ensure that only "fit" transcripts exit the nucleus and are translated. Targeted mRNA turnover is integral to this process. Finally, newly discovered means of antisense regulation, including RNA interference, rely on selective mRNA turnover for their biological consequences. For these reasons, mRNA turnover is attracting heightened interest from all areas of the life sciences. The proposed FASEB conference is unique in concentrating on mRNA decay, thereby exploring in diverse biological settings the mechanisms, both at the cellular and molecular levels, that underpin this universal process. The proposed meeting is particularly noteworthy in attracting investigators who study pro- and eukaryotic organisms and in focussing on mechanisms by which RNAs interact with the decay apparatus and the factors which modulate decay. The first third of the conference will explore the latest developments in our understanding of how the enzymes of mRNA decay initially interact with their targets. Such interactions largely occur at the ends of RNAs, often involving highly conserved proteins or protein complexes, but with many surprising twists. We anticipate that much needed structural investigations of these interactions will come to fruition and be presented for the first time at the proposed conference. The second portion of the conference will focus on the complex interactions among nascent mRNAs, the degradative machinery, nuclear export, quality control and translation. Understanding these processes and their interplay has myriad implications for regulation of growth, development, and disease. The final portion of the conference will focus on mechanisms of RNA interference (as opposed to the phenomenology) and the application of global approaches to understanding mRNA decay and its impact on whole cells. The conference will provide a superb overview of the latest progress in an exciting field and will propel the next stage of its development.

描述（由申请人提供）：我们正在请求对FASEB会议的部分支持，该会议将于2004年6月26日至7月1日在亚利桑那州图森市举行，主题为“基因表达的转录后调节：mRNA衰变机制”。 mRNA的周转是基因表达的关键但经常未被识别的调节因子，因为mRNA的稳定性决定了其最终的稳态水平。 此外，程序性不稳定性是抑制基因表达的重要机制。 此外，越来越多的人认识到，大多数生物体采用监测mRNA质量的手段，以确保只有“合适”的转录本离开细胞核并被翻译。 靶向mRNA周转是这一过程的组成部分。 最后，新发现的反义调控手段，包括RNA干扰，依赖于选择性mRNA周转的生物学后果。 由于这些原因，mRNA周转率正引起生命科学所有领域的高度关注。 拟议的FASEB会议是独一无二的，集中在mRNA衰变，从而探索在不同的生物环境中的机制，无论是在细胞和分子水平上，支持这一普遍的过程。 拟议的会议是特别值得注意的吸引研究人员谁研究亲和真核生物，并在集中在机制，其中RNA相互作用的衰变装置和因素，调节衰变。 会议的前三分之一将探讨我们对mRNA衰变酶最初如何与其靶点相互作用的理解的最新进展。 这种相互作用主要发生在RNA的末端，通常涉及高度保守的蛋白质或蛋白质复合物，但有许多令人惊讶的曲折。 我们预计，这些相互作用急需的结构研究将取得成果，并首次在拟议的会议上提出。 会议的第二部分将重点关注新生mRNA之间的复杂相互作用，降解机制，核出口，质量控制和翻译。 了解这些过程及其相互作用对生长、发育和疾病的调节有着无数的意义。 会议的最后一部分将集中在RNA干扰的机制（而不是现象学）和全球方法的应用，以了解mRNA衰变及其对整个细胞的影响。 会议将提供一个令人兴奋的领域的最新进展的极好概述，并将推动其发展的下一阶段。