Post-transcriptional control of gene expression

基因表达的转录后控制

• 批准号: 6761390
• 负责人: ROY PARKER
• 金额: $ 0.3万
• 依托单位: FEDERATION OF AMER SOC FOR EXPER BIOLOGY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-15 至 2005-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6761390
• 关键词: gene expression; meeting /conference /symposium; posttranscriptional RNA processing; travel

DESCRIPTION (provided by applicant): We are requesting partial support for a FASEB Conference on "post-transcriptional regulation of gene expression: mechanisms of mRNA decay" to be held in Tucson AZ on June 26-July 1, 2004. Turnover of mRNA is a key, but frequently unrecognized, regulator of gene expression as the stability of an mRNA dictates its ultimate steady-state level. Moreover, programmed instability is an important mechanism of repressing gene expression. In addition, it is increasingly recognized that most organisms employ means of surveillance of mRNA quality to ensure that only "fit" transcripts exit the nucleus and are translated. Targeted mRNA turnover is integral to this process. Finally, newly discovered means of antisense regulation, including RNA interference, rely on selective mRNA turnover for their biological consequences. For these reasons, mRNA turnover is attracting heightened interest from all areas of the life sciences. The proposed FASEB conference is unique in concentrating on mRNA decay, thereby exploring in diverse biological settings the mechanisms, both at the cellular and molecular levels, that underpin this universal process. The proposed meeting is particularly noteworthy in attracting investigators who study pro- and eukaryotic organisms and in focussing on mechanisms by which RNAs interact with the decay apparatus and the factors which modulate decay. The first third of the conference will explore the latest developments in our understanding of how the enzymes of mRNA decay initially interact with their targets. Such interactions largely occur at the ends of RNAs, often involving highly conserved proteins or protein complexes, but with many surprising twists. We anticipate that much needed structural investigations of these interactions will come to fruition and be presented for the first time at the proposed conference. The second portion of the conference will focus on the complex interactions among nascent mRNAs, the degradative machinery, nuclear export, quality control and translation. Understanding these processes and their interplay has myriad implications for regulation of growth, development, and disease. The final portion of the conference will focus on mechanisms of RNA interference (as opposed to the phenomenology) and the application of global approaches to understanding mRNA decay and its impact on whole cells. The conference will provide a superb overview of the latest progress in an exciting field and will propel the next stage of its development.

描述（由申请人提供）：我们请求对将于 2004 年 6 月 26 日至 7 月 1 日在亚利桑那州图森举行的关于“基因表达转录后调控：mRNA 衰减机制”的 FASEB 会议提供部分支持。mRNA 的周转是基因表达的一个关键但经常未被认识到的调节因子，因为 mRNA 的稳定性决定了其最终的稳态水平。 此外，程序性不稳定性是抑制基因表达的重要机制。 此外，人们越来越认识到，大多数生物体都采用 mRNA 质量监控手段来确保只有“适合”的转录本离开细胞核并进行翻译。 靶向 mRNA 周转是这一过程不可或缺的一部分。 最后，新发现的反义调节方法（包括 RNA 干扰）依赖于选择性 mRNA 更新来产生生物学后果。 由于这些原因，mRNA 周转引起了生命科学各个领域的高度关注。 拟议的 FASEB 会议的独特之处在于专注于 mRNA 衰变，从而在不同的生物环境中探索支撑这一普遍过程的细胞和分子水平机制。 拟议的会议特别值得注意的是，它吸引了研究原核生物和真核生物的研究人员，并重点关注 RNA 与衰变装置相互作用的机制以及调节衰变的因素。 会议的前三分之一将探讨我们对 mRNA 衰变酶最初如何与其靶标相互作用的理解的最新进展。 这种相互作用主要发生在 RNA 末端，通常涉及高度保守的蛋白质或蛋白质复合物，但也有许多令人惊讶的扭曲。 我们预计，对这些相互作用急需的结构性研究将取得成果，并在拟议的会议上首次提出。 会议的第二部分将重点关注新生 mRNA、降解机制、核输出、质量控制和翻译之间复杂的相互作用。 了解这些过程及其相互作用对于生长、发育和疾病的调节具有多种意义。 会议的最后部分将重点讨论 RNA 干扰机制（而不是现象学）以及应用全局方法来了解 mRNA 衰减及其对整个细胞的影响。 会议将对这一令人兴奋的领域的最新进展进行精彩概述，并将推动其下一阶段的发展。