Spine Dynamics Underlying Ocular Dominance Plasticity

眼部优势可塑性背后的脊柱动力学

• 批准号: 6936315
• 负责人: SPENCER LAVERE SMITH
• 金额: $ 4.21万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6936315
• 关键词: brain electrical activity; brain imaging /visualization /scanning; brain mapping; cerebral dominance; confocal scanning microscopy; dendrites; developmental neurobiology; genetically modified animals; laboratory mouse; long term potentiation; neural plasticity; neural transmission; neuronal guidance; postdoctoral investigator; single cell analysis; synaptogenesis; time resolved data; visual cortex; visual deprivation

DESCRIPTION (provided by applicant): In the developing nervous system, initially promiscuous synaptic connections are remodeled by activity-dependent mechanisms, with axons strengthening their connections with some targets while completely disconnecting from others. In the neocortex, this process has been most thoroughly studied in the developing primary visual cortex, where decorrelating the activity of the two eyes dramatically reduces cortical binocularity. These functional changes in cortical physiology are widely accepted to be anchored in long-lasting changes in the efficacy of synaptic connections. However, the specific mechanisms by which synaptic plasticities lead to changes in the function of cortical circuitry remain elusive. The major objective of the work proposed here is to determine where in the cortical circuit experience-dependent changes in binocular responses are first observed, track the progression over time, and test the hypothesis that these changes are anchored in anatomical changes in synaptic connectivity. To address these questions, techniques such as 2-photon laser scanning microscopy, intrinsic signal optical imaging, and single-unit recordings, will be used to map circuit plasticity and follow changes in synaptic connectivity in vivo.

描述（由申请人提供）：在发育中的神经系统中，最初混杂的突触连接通过活性依赖性机制重塑，轴突加强其与某些靶点的连接，同时与其他靶点完全断开。在新皮层中，这一过程在发育中的初级视觉皮层中得到了最彻底的研究，在那里，双眼活动的去相关极大地减少了皮层的双眼性。皮质生理学中的这些功能变化被广泛接受为锚定在突触连接功效的持久变化中。然而，突触可塑性导致皮层电路功能变化的具体机制仍然难以捉摸。这里提出的工作的主要目标是确定在皮层电路的经验依赖的变化，双眼反应首先观察到，跟踪随着时间的推移的进展，并测试的假设，这些变化是锚定在突触连接的解剖变化。为了解决这些问题，技术，如2-光子激光扫描显微镜，内在信号光学成像，和单单位记录，将被用来映射电路可塑性，并遵循体内突触连接的变化。