The role of HNF4 in gastrointestinal development

HNF4在胃肠道发育中的作用

• 批准号: 6914445
• 负责人: MICHELE A BATTLE
• 金额: $ 4.83万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6914445
• 关键词: cell differentiation; chromatin immunoprecipitation; electron microscopy; embryogenesis; gastrointestinal epithelium; gastrointestinal system; gene expression; genetically modified animals; histogenesis; histology; hormone receptor; immunocytochemistry; laboratory mouse; liver; liver cells; microarray technology; polymerase chain reaction; postdoctoral investigator; transcription factor

DESCRIPTION (provided by applicant): The goals of the proposed studies are to determine if HNF4 is essential for gastrointestinal development, if HNF4 is a central regulator of gastrointestinal gene expression, and if HNF4 controls gene expression by modulating the receptivity of promoters/enhancers to transcriptional activators. Previous studies have shown that HNF4 is a key mediator of gene expression in the liver and that its elimination in the liver blocks hepatocyte differentiation. Cre-loxP technology will be used to conditionally remove Hnf4 from the epithelial cells of the mouse gastrointestinal tract during embryogenesis. The effect of loss of HNF4 in the duodenum, the jejunum and ileum, and the transverse colon will be examined using a combination of histological, immunohistological, and molecular techniques. Gene arrays will be used to identify genes whose expression is dependent upon HNF4 in the gut. Chromatin immunoprecipitation analyses will be used to identify changes in the set of factors bound to gut-specific promoters/enhancers when HNF4 is eliminated from gut epithelial cells. By understanding how this transcription factor functions in the gut, greater insight into the fundamental molecular processes underlying gut development wilt be gained.

描述（由申请人提供）：