Novel Strategies to Improve Yeast as an Expression Platform

巴斯德毕赤酵母(重新分类为Komagataella Pastoris)，是生物制药行业中成熟的蛋白质表达平台。它的价值在于它有强大的和严格控制的启动子的可用性，它生长到非常高的密度，以及它每升培养物分泌克量重组蛋白的能力。然而，仍然有改进的潜力，特别是当一些感兴趣的蛋白质在这个系统中很难表达的时候。该项目旨在开发旨在提高产品质量和效价的新策略。这是对Fujifilm DiSynth BioTechnologies(FDB)提出的挑战的回应，目的是改进他们的毕赤酵母表达系统，这是他们广泛能力的一部分，能够执行从基因到最终重组蛋白质原料药的合同工作。它将被纳入生物处理英才中心2.0，这是爱丁堡大学、曼彻斯特大学和约克大学、IBioIC和FDB之间的新伙伴关系。这是一系列协作的跨学科研究项目，旨在应对生物制剂制造中的挑战。

Pichia pastoris (reclassified as Komagataella pastoris), is an established protein expression platform in the biopharmaceutical industry. It is valued for the availability of powerful and tightly regulated promoters, for its growth to very high densities and for its ability to secrete gram amounts of recombinant protein per litre of culture. Nevertheless, there is potential for improvement, especially as some proteins of interest are difficult to express in this system. This project seeks to develop novel strategies aimed at improving product quality and titre. It is a response to a challenge from Fujifilm Diosynth Biotechnologies (FDB) to improve their Pichia expression system, part of their broad range of capabilities enabling contract work to be performed from gene to final recombinant protein bulk drug substance. It will be encompassed within the Centre of Excellence in Bioprocessing 2.0, a new partnership between the Universities of Edinburgh, Manchester and York, the IBioIC and FDB. This is a rolling series of collaborative interdisciplinary research projects to address challenges in the manufacture of biologics.