Micromachined Si Printhead for Microarray Fabrication

用于微阵列制造的微机械硅打印头

• 批准号: 6995025
• 负责人: ROBERT C HAUSHALTER
• 金额: $ 31.51万
• 依托单位: PARALLEL SYNTHESIS TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6995025
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; biotechnology; gene expression; high throughput technology; microarray technology; printing; silicon

DESCRIPTION (provided by applicant): During the Phase I effort, a totally new type of passive microcontact printing tool was developed by Parallel Synthesis Technologies that can print microarrays of a quality, cost and speed previously unobtainable. Silicon micromachining techniques were employed to fabricate a robust, inexpensive and very high density "printing microtiter plate" which prints sub-nL individual samples from each well onto a microarray format. The technology is based on arrays (1536 or 6144 SBS format) of individually compliant, silicon-spring loaded, hollow bore nozzles, with print tips in the range of 25-1 OOum connected to reservoir wells of volume about 0-5 uL, which are filled by automated liquid dispensers. Very large numbers (up to 10,000 spots/tip/loading) of extremely uniform spots (%CV spot size for one pin approximately 2% and 100 pins is approximately 5%) can be rapidly printed at an estimated rate of 1-10 x 10^6 spots/hour/head with less than <0.07% missing spots. In Phase II, two print heads of full microscope slide size (25 x 75 mm) will be fabricated at 1536 to 6144 formats to give a total of 300 or 1200 tips/print head, respectively. A proposed printer design, where one of these arrays is selected from among many in a carousel, should allow microarray printing at an unprecedented rate. After fabrication and testing at Parallel, the print heads will be distributed to three collaborators to determine if this technology will produce DNA and protein microarrays of an improved quality in three different array fabrication settings. The parallel print head will be employed (a) to print a 44,000 spot viral DNA array in an academic research setting with Prof. J. DeRisi (UCSF), (b) to print several hundred spot prototype diagnostic arrays with Dr. T. Tisone (Biodot, Inc.) and (c) to print antibody arrays in microtiter plate formats with Dr. D. Bochkariov (BD - Clontech). If these printing tools provide the anticipated large improvements, then the testing, sale and distribution can employ the existing channels currently used by Parallel for its commercially successful Silicon Microarray(tm) technology based on individual silicon pins which has been very well received by the microarray community. A fundamentally new type of multilevel silicon etch process was discovered during the course of this work and a patent application is in preparation.

描述（由申请人提供）：在第一阶段的努力，一个全新类型的被动微接触印刷工具是由平行合成技术，可以打印微阵列的质量，成本和速度以前无法获得。采用硅微机械加工技术来制造坚固、廉价和非常高密度的“印刷微量滴定板”，其将来自每个孔的sub-nL个体样品印刷到微阵列格式上。该技术基于单独顺应的、硅弹簧加载的、中空孔喷嘴的阵列（1536或6144 SBS格式），其中25- 100 μ m范围内的打印头连接到约0-5 μ L体积的储存器威尔斯孔，其由自动液体分配器填充。可以以1-10 x 10^6个点/小时/头的估计速率快速打印大量（高达10，000个点/针尖/装载）极其均匀的点（1个针的%CV点大小约为2%，100个针的%CV点大小约为5%），缺失点小于0.07%。在第二阶段，两个完整的显微镜载玻片尺寸（25 x 75 mm）的打印头将在1536至6144格式制造，以分别提供300或1200个尖端/打印头。一个拟议的打印机设计，其中一个这些阵列是从许多在一个旋转木马中选择，应该允许以前所未有的速度打印微阵列。在平行制造和测试之后，打印头将被分发给三个合作者，以确定这项技术是否会在三种不同的阵列制造环境中生产质量更高的DNA和蛋白质微阵列。平行打印头将用于（a）与J. DeRisi教授（UCSF）在学术研究环境中打印44，000个点的病毒DNA阵列，（B）与T. Tisone（Biodot，Inc.）和（c）与D. Bochkariov（BD - Clontech）.如果这些打印工具提供了预期的大的改进，那么测试、销售和分销可以采用目前由Parallel用于其商业上成功的基于单个硅引脚的硅微阵列（TM）技术的现有渠道，该技术已经被微阵列社区很好地接受。 在这项工作的过程中发现了一种全新类型的多级硅蚀刻工艺，专利申请正在准备中。