Ultra-High Throughput, High Purity Stem Cell Isolation

超高通量、高纯度干细胞分离

• 批准号: 6881884
• 负责人: MANISH DESHPANDE
• 金额: $ 71.08万
• 依托单位: CYTONOME, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-15 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6881884
• 关键词: CD34 molecule; bioengineering /biomedical engineering; cell sorting; consumable /disposable biomedical equipment; data collection methodology /evaluation; fluid flow; hematopoietic stem cells; high throughput technology; optics; stem cells; technology /technique development; tissue /cell preparation

DESCRIPTION (provided by applicant): There is considerable evidence for the application of transplantation of Hematopoietic and Mesenchymal Stem Cells in the treatment of a wide range of cancers including lymphoma, neuroblastoma, and multiple myeloma. In addition, HSC/MSC therapies can be applied to reconstitute the immune system, regenerate damaged tissues, and deliver genes to repair damaged cells for many auto-immune diseases such as diabetes, rheumatoid arthritis, and systemic lupus erythematosis. One of the major roadblocks in the successful application of transplant treatments is the difficulty in rapidly obtaining a pure sample of stem cells in the harvested sample. This ultimate objective of our research is the development of an ultra-high throughput high-purity cell sorter, Gigasort, for stem cell harvesting. The cell sorter consists of several parallel microfluidic channels that combine to produce throughput that is expected to be at least 10x that of current generation cell sorters in practice, without compromising sample purity. The current proposal is aimed at developing a multichannel sorter test bed that is extendable in the number of parallel channels used. We will build the test bed and experimentally measure the sorting performance for fluorescent beads and cells, including cells harvested from blood.

描述（由申请人提供）：有大量证据表明造血干细胞和间充质干细胞移植可用于治疗多种癌症，包括淋巴瘤、神经母细胞瘤和多发性骨髓瘤。此外，HSC/MSC疗法可用于重建免疫系统，再生受损组织，并递送基因以修复许多自身免疫疾病（如糖尿病，类风湿性关节炎和系统性狼疮性肾炎）的受损细胞。移植治疗成功应用的主要障碍之一是难以在收获的样品中快速获得干细胞的纯样品。 我们研究的最终目标是开发用于干细胞收获的超高通量高纯度细胞分选仪Gigasort。细胞分选仪由几个平行的微流体通道组成，这些通道联合收割机在实践中产生的通量预计至少是当前一代细胞分选仪的10倍，而不影响样品纯度。目前的建议是旨在开发一个多通道分拣机测试床，是可扩展的并行通道使用的数量。我们将建立测试床，并通过实验测量荧光珠和细胞（包括从血液中采集的细胞）的分选性能。