Hierarchical Sorter for High Purity Stem Cell Selection

用于高纯度干细胞选择的分层分选机

• 批准号: 6789056
• 负责人: MANISH DESHPANDE
• 金额: $ 10万
• 依托单位: CYTONOME, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6789056
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; cell sorting; cell transplantation; clinical biomedical equipment; hematopoietic stem cells; immunotherapy; neoplasm /cancer therapy; stem cell transplantation; stem cells; tissue /cell preparation

DESCRIPTION (provided by applicant): There is considerable evidence for the application of transplantation of Hematopoietic and Mesenchymal Stem Cells in the treatment of a wide range of cancers including lymphoma, neuroblastoma, and multiple myeloma. In addition, HSC/MSC therapies can be applied to reconstitute the immune system, regenerate damaged tissues, and deliver genes to repair damaged cells for many auto-immune diseases such as diabetes, rheumatoid arthritis, and system lupus erythematosis. One of the major roadblocks in the successful application of transplant treatments is the difficulty in obtaining a pure sample of stem cells in the harvested sample. This results in reinfusion of a larger number of residual tumor cells into the patient than is desirable, resulting, in some cases, in relapse of the disease. The ability to acquire a pure stem cell sample is therefore highly desirable. This research is focussed specifically on the development of a high-purity cell sorted for stem cell harvesting. The cell sorter uses a hierarchical multistage strategy with each sorting stage operating on the results of the previous sort thus reintorraging a sorted sample to verify its accuracy. The overall objective of this research is a sorter that results in a sample with greater than 99.9% purity through the use of multiple sorts.

描述（由申请人提供）：有大量证据表明造血干细胞和间充质干细胞移植在治疗多种癌症中的应用，包括淋巴瘤、神经母细胞瘤和多发性骨髓瘤。此外，HSC/MSC疗法可用于重建免疫系统，再生受损组织，并为许多自身免疫性疾病（如糖尿病，类风湿性关节炎和系统性狼疮红斑病）提供修复受损细胞的基因。成功应用移植治疗的主要障碍之一是难以在收获的样本中获得纯干细胞样本。这导致大量残留肿瘤细胞回流到患者体内，在某些情况下导致疾病复发。因此，获得纯干细胞样本的能力是非常可取的。