Automated Microchannel Bovine Culture Parameter Studies

自动化微通道牛培养参数研究

• 批准号: 6844763
• 负责人: KATHRYN HAUBERT
• 金额: $ 29.25万
• 依托单位: VITAE, LLC
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-22 至 2007-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6844763
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical automation; biomedical equipment development; cow; egg /ovum; embryo /fetus culture; growth media; in vitro fertilization; miniature biomedical equipment

DESCRIPTION (provided by applicant): An important limitation of current IVP methods is the ability to perform experiments involving large numbers of embryos and repetitions in a highly accurate and repeatable way. Current methods are laborious, inefficient and depend on the skill of the technician. Micro channel embryo culture enables automated high throughput processing because the embryo is not moved, but rather held in a "parking place." This allows the fluid to be exchanged via automated systems rather than attempting to automate embryo manipulation. We propose to develop an automated micro channel embryo culture system to allow rapid optimization of micro channel culture conditions. The system utilizes commercially available automated liquid handling equipment combined with a custom incubation chamber and a digital visualization system. Specific advantages of the proposed automated micro channel culture system (AMCS) include the use of micro channel culture devices (Vitae Cell TM) which facilitates automated processing and allows one to achieve gentle medium changes, a closed system that eliminates perturbations in gas concentrations and temperature, robotically controlled medium changes and visualization eliminating operator variability. The system will initially be used to optimize bovine embryo culture. We propose to investigate parameters such as medium (single vs. sequential), medium replenishment (by flow), gas atmosphere, embryo-to-volume ratio, and medium supplementation in the micro channel system compared to the standard micro-drop.

描述(由申请人提供)：当前IVP方法的一个重要限制是无法以高度准确和可重复的方式进行涉及大量胚胎和重复的实验。目前的方法费力、低效，而且依赖于技术人员的技能。微通道胚胎培养能够实现自动化的高通量处理，因为胚胎不会被移动，而是被保存在“停车位”。这使得液体可以通过自动化系统进行交换，而不是试图使胚胎操作自动化。我们建议开发一种自动化的微通道胚胎培养系统，以便快速优化微通道培养条件。该系统利用商业上可获得的自动化液体处理设备，结合定制孵化室和数字可视化系统。建议的自动微通道培养系统(AMCS)的具体优势包括使用微通道培养设备(Vitae Cell TM)，它促进了自动化处理并允许实现温和的介质更换，消除了气体浓度和温度扰动的封闭式系统，机器人控制的介质更换，以及消除操作员变异性的可视化。该系统最初将用于优化牛胚胎培养。我们建议研究微通道系统中的介质(单次与顺序)、介质补充(通过流动)、气体气氛、胚胎体积比和介质补充等参数，并与标准微滴进行比较。