Cardiac Channel Mutations in SIDS

SIDS 中的心脏通道突变

• 批准号: 6904585
• 负责人: MICHAEL JOHN ACKERMAN
• 金额: $ 39.51万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-07 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6904585
• 关键词: arrhythmia; calcium channel; clinical research; disease /disorder etiology; electrophysiology; gene expression; gene mutation; genetic polymorphism; heart disorder; high performance liquid chromatography; high throughput technology; human tissue; infant human (0-1 year); long QT syndrome; myocardium; nucleic acid sequence; postmortem; potassium channel; site directed mutagenesis; sodium channel; sudden infant death syndrome; voltage /patch clamp

DESCRIPTION (provided by applicant): Michael J. Ackerman, M.D., Ph.D. is a board eligible pediatric cardiologist and an Assistant Professor of Medicine, Pediatrics, and Molecular Pharmacology & Experimental Therapeutics at Mayo Medical School. Dr. Ackerman is a physician-scientist directing a sudden death genomics laboratory and the Long QT Syndrome Clinic. His long-term objectives are to identify the underlying causes of sudden cardiac death in infants, children, adolescents, and young adults. In this proposal entitled Cardiac Channel Mutations in Sudden Infant Death Syndrome, the applicant sets forth to answer the fundamental question: what percentage of infants suffering a SIDS death possessed putative disease-causing mutations in the genes encoding their cardiac ion channels? Presently, SIDS continues to claim nearly 3000 apparently healthy infants each year in the United States. The fundamental causes underlying SIDS remain poorly understood. In specific aim 1, Dr. Ackerman will perform a mutational analysis of the 5 cardiac channel genes already implicated in a human arrhythmia syndrome, namely congenital long QT syndrome using temperature modulated heteroduplex analysis and denaturing high performance liquid chromatography. In specific aim 2, two non-LQTS arrhythmia syndrome ion channel genes will be investigated by mutational analysis as novel candidate SIDS genes. Finally, in specific aim 3, the possible mutations in the channel genes identified in aims 1 and 2 will be characterized functionally. These mutations will be engineered by site-directed mutagenesis into the wild type channel, expressed in transient and stable transfection cell lines, and characterized using single electrode patch clamp technologies. If the applicant's hypothesis is correct that 10% of infants with SIDS possess cardiac channel mutations, then cardiac channel genes would account for the "underlying vulnerability" for the largest identifiable subset of infants to date. Such a discovery could have significant implications on attempts to further reduce the incidence of SIDS in our country and throughout the world.

描述（由申请人提供）：Michael J.阿克曼，医学博士，博士是一名合格的儿科心脏病专家，也是马约医学院医学、儿科、分子药理学和实验治疗学的助理教授。阿克曼博士是一位医生兼科学家，负责指导一个猝死基因组学实验室和长QT综合征诊所。他的长期目标是确定婴儿、儿童、青少年和年轻人心脏性猝死的根本原因。在这项题为“婴儿猝死综合征中的心脏通道突变”的提案中，申请人提出了一个基本问题的答案：患有SIDS死亡的婴儿中有多大比例的婴儿在编码其心脏离子通道的基因中具有推定的致病突变？目前，SIDS继续声称在美国每年有近3000名明显健康的婴儿。对小岛屿发展中国家的根本原因仍然知之甚少。在具体目标1中，阿克曼博士将使用温度调节异源双链分析和变性高效液相色谱法，对已经与人类心律失常综合征（即先天性长QT综合征）有关的5种心脏通道基因进行突变分析。在具体目标2中，将通过突变分析研究两个非LQTS心律失常综合征离子通道基因作为新的候选SIDS基因。最后，在具体目标3中，将在功能上表征目标1和2中鉴定的通道基因中的可能突变。这些突变将通过定点诱变工程化到野生型通道中，在瞬时和稳定转染细胞系中表达，并使用单电极膜片钳技术表征。如果申请人的假设是正确的，即10%的SIDS婴儿具有心脏通道突变，那么心脏通道基因将解释迄今为止最大的可识别婴儿子集的“潜在脆弱性”。这一发现可能对进一步减少我国和全世界小岛屿发展中国家发病率的努力产生重大影响。