Tuneable multifocal plane microscopy for high frame rate three-dimensional fluorescence microscopy of cilia/flagella

用于纤毛/鞭毛高帧率三维荧光显微镜的可调谐多焦点平面显微镜

• 批准号: 2446518
• 金额: --
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2446518
• 关键词: Tuneable; multifocal; plane; microscopy; frame

Flagella and cilia are protrusion from cell surfaces with motility, signalling and sensory functions, possessing a highly conserved molecular skeleton found across eukaryotic life. One useful model of flagellar motility is Leishmania mexicana (L. mexicana), a protozoan parasite possessing a single motile flagellum. L. mexicana is one of the most diverged eukaryotes, and the aetiological agent of leishmaniasis, a neglected tropical disease. The flagellum has a multitude of important roles, extending beyond cell motility to morphogenesis, vector attachment and, potentially, sensory reception. Therefore, studying flagella in this model organism allows the investigation of questions relating to both eukaryotic-wide and parasite-specific flagellar biology. This project aims to elucidate structural and functional aspects of flagellar biology by developing new microscopy techniques in collaboration with Cairn Research Institute to visualise the flagella in swimming L. mexicana cells.BBSRC priority areas the proposed research addressed:World-Class Underpinning BioscienceBioscience for HealthExploiting New Ways of Working

鞭毛和纤毛是细胞表面的突起，具有运动、信号和感觉功能，具有高度保守的分子骨架，广泛存在于真核生物中。鞭毛运动的一个有用模型是墨西哥利什曼原虫(L.miciana)，一种拥有单一活动鞭毛的原生动物寄生虫。墨西哥利什曼原虫是最具多样性的真核生物之一，也是一种被忽视的热带疾病--利什曼病的病原体。鞭毛有许多重要的作用，从细胞运动延伸到形态发生、载体附着和潜在的感觉接收。因此，在这种模式生物中研究鞭毛，可以研究与真核生物范围和寄生虫特有的鞭毛生物学有关的问题。该项目旨在通过与凯恩研究所合作开发新的显微镜技术来显示游泳的墨西哥乳杆菌细胞中的鞭毛，以阐明鞭毛生物学的结构和功能方面。BBSRC建议的研究重点领域：世界级基础生物科学促进健康的生物科学探索新的工作方法