Equipment Core

核心B-“设备核心”。“设备核心”支持主要设备，用于表征计划项目的各种项目的蛋白质系统的动态性质。支持两种光谱方法： 1.温度跃迁弛豫光谱。在三个重叠的时间域中，使用三个仪器覆盖从10ps到几分钟(或更长)的整个时间范围。 (A)10ps-10 ns。T跳跃是通过激光照射加热水来实现的，仪器的分辨率是由吸收能量的热化时间来设置的。这台皮秒仪器位于洛斯阿拉莫斯。红外和紫外/可见吸收光谱和发射光谱都可以作为蛋白质结构的探针。 (B)15 ns-10ms。T跳跃是通过激光照射加热水来实现的。三个独立的 将使用分光计。位于阿尔伯特·爱因斯坦的两台仪器可以进行紫外/可见光吸收和/或荧光。红外线瞬变研究将在洛斯阿拉莫斯进行。 (C)1毫秒-分钟(或更长)。这台仪器将在爱因斯坦公司购买并安装。T跳跃是通过在不同温度下混合两种溶液并将混合溶液保持在最终温度来实现的。使用光学(UV/Vis)发射和/或吸收光谱和红外吸收光谱作为结构探针。 2.位于阿尔伯特·爱因斯坦的静态同位素编辑红外差谱仪。一般而言，静态温度测量先于T跳跃动力学研究。它们校准了动力学工作中要观察到的预期信号大小，并在动力学实验中设置了无限时间的信号大小。

Core B - 'The Equipment Core'. The 'Equipment Core' supports the major equipment used to characterized the dynamical nature of the protein systems of the various projects of the Program Project. Two spectroscopic approaches are supported: 1. Temperature-jump relaxation spectroscopy. Three instruments are employed to cover the entire time range from 10 ps to minutes (or longer) in three overlapping time domains. (a) 10 ps - 10 ns. The T-jump is performed by heating water by laser irradiation, and the resolution of the instrument is set by the thermalization time of the absorbed energy. This picosecond instrument is located at Los Alamos. Both IR and UV/Vis absorbance and emission can be performed as probes of protein structure. (b) 15 ns-10 ms. The T-jump is achieved by heating water by laser irradiation. Three separate spectrometers will be employed. Two instruments are located at Albert Einstein and perform UV/Vis absorbance and/or fluorescence. IR transient studies will be performed at Los Alamos. (c) 1 ms - minutes (or longer). This instrument will be purchased and based at Einstein. The T-jump is achieved by mixing two solutions at different temperatures and holding the mixed solution at a final temp. Optical (UV/Vis) emission and or absorbance and IR absorbance spectroscopies are employed as structural probes. 2. A static isotope editing IR difference spectrometer located at Albert Einstein. In general, static thermal measurements precede T-jump kinetic studies. These calibrate the expected signal size to be observed in the kinetic work and also sets the magnitude of signal at infinite time in the kinetics experiments.