Developing new tools to investigate complex redox-active proteins

开发新工具来研究复杂的氧化还原活性蛋白

• 批准号: 2453516
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2453516
• 关键词: Developing; new; tools; investigate; complex

I intend to investigate the elusive mechanism of respiratory enzyme complex I, a protein that is essential for ATP synthesis and whose malfunction is associated with ageing and numerous neurodegenerative disorders including Parkinson's disease. In order to acquire cutting-edge insight into large metalloenzymes such as the complex I, a novel technique developed by Roessler lab will be applied and developed further, which involves the combination of electron paramagnetic resonance (EPR) spectroscopy and protein film electrochemistry (PFE).

我打算研究呼吸酶复合物I的难以捉摸的机制，呼吸酶复合物I是ATP合成所必需的蛋白质，其功能障碍与衰老和许多神经退行性疾病（包括帕金森病）有关。为了获得对大型金属酶（如复合物I）的前沿见解，Roessler实验室开发的一种新技术将得到进一步应用和发展，该技术涉及电子顺磁共振（EPR）光谱和蛋白质膜电化学（PFE）的结合。