Human Metapneumovirus Infection in Adults

成人人类偏肺病毒感染

• 批准号: 6984917
• 负责人: Ann R Falsey
• 金额: $ 22.56万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-15 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6984917
• 关键词: Paramyxoviridae; Paramyxoviridae disease; clinical research; human data; human middle age (35-64); human old age (65+); human tissue; immune response; immunologic assay /test; laboratory mouse; monoclonal antibody; polymerase chain reaction; respiratory disorder epidemiology; respiratory infections; virus classification

DESCRIPTION (provided by applicant): Human metapneumovirus (hMPV) is a newly discovered respiratory virus and may be an unrecognized cause of serious respiratory illness in elderly adults. Our preliminary data suggests that hMPV may account for a substantial number of hospitalizations in this age group, and therefore is deserving of in depth study, possibly with efforts toward vaccine development. At present there is little available information about the epidemiology and clinical impact of hMPV in adult age groups. As a first step towards a better understanding of the importance of hMPV in adult populations, serum samples, nasal secretion specimens and clinical data from a recently completed 4-year prospective surveillance study of respiratory infections in several cohorts of adults will be analyzed for hMPV. We have developed sensitive and specific real time RT-PCR as well as serum enzyme immunoassay (ElA) for diagnosis of hMPV. Overall, 1575 young adults, healthy elderly, high-risk adults and residents of long term care facilities were followed during a 4 year period from 1999 - 2003. These subjects had 1428 illnesses evaluated, of which 95% are available for hMPV analysis. In addition, 1330 hospitalized subjects were evaluated during this period. Overall, 214 influenza A and 294 RSV infections were identified. Pre- and post-season sera from prospective cohorts and acute and convalescent sera from all illnesses will be analyzed for hMPV infection using an EIA. All nasal secretions from acute illnesses will be tested for hMPV by RT-PCR. The incidence of symptomatic and asymptomatic hMPV infection will be calculated and compared with influenza and RSV infections identified in these subjects during the same period. The clinical characteristics, including clinical impact, of hMPV illnesses in each of the adult subgroups will be analyzed and compared with RSV and influenza. hMPV will be identified as group A or B by strain specific RT-PCR and the epidemiology and clinical impact of each virus strain will be described. In addition to determining the incidence and impact of hMPV in adult populations we will analyze the role of serum and mucosal antibodies in protection from hMPV infection and severe disease. Monoclonal antibodies (Mabs) to HMPV will be generated to facilitate development of a serum micro neutralization assay for the study of functional antibody. This study will provide data that will be useful in determining the need for and design of future larger scale prospective studies of HMPV in the elderly as well as the feasibility of vaccines based on stimulating humeral immunity.

描述（由申请方提供）：人偏肺病毒（hMPV）是一种新发现的呼吸道病毒，可能是老年人严重呼吸道疾病的未被识别的原因。我们的初步数据表明，hMPV可能是该年龄组住院的主要原因，因此值得深入研究，可能需要努力开发疫苗。目前，关于成人年龄组中hMPV的流行病学和临床影响的可用信息很少。作为更好地了解hMPV在成人人群中重要性的第一步，将对最近完成的几个成人队列中呼吸道感染4年前瞻性监测研究的血清样本、鼻分泌物标本和临床数据进行hMPV分析。本研究建立了灵敏、特异的真实的时间RT-PCR和血清酶免疫分析法（ElA）。总体而言，1575年轻人，健康的老年人，高风险的成年人和长期护理设施的居民在1999年至2003年的4年期间进行了跟踪。这些受试者评价了1428种疾病，其中95%可用于hMPV分析。此外，在此期间对1330例住院受试者进行了评价。总体而言，确定了214例甲型流感和294例RSV感染。将使用EIA分析来自前瞻性队列的季节前和季节后血清以及来自所有疾病的急性和恢复期血清的hMPV感染。所有急性疾病的鼻分泌物将通过RT-PCR检测hMPV。将计算症状性和无症状性hMPV感染的发生率，并与同期在这些受试者中确定的流感和RSV感染进行比较。将分析每个成人亚组中hMPV疾病的临床特征（包括临床影响），并与RSV和流感进行比较。将通过毒株特异性RT-PCR将hMPV鉴定为A组或B组，并将描述每种病毒毒株的流行病学和临床影响。除了确定hMPV在成人人群中的发病率和影响外，我们还将分析血清和粘膜抗体在预防hMPV感染和严重疾病中的作用。将生成HMPV单克隆抗体（Mab），以促进血清微量中和试验的开发，用于功能性抗体的研究。这项研究将提供的数据，将是有用的，在确定需要和设计未来更大规模的前瞻性研究HMPV在老年人以及疫苗的可行性的基础上刺激肱骨免疫。