Elucidating the signalling pathways important for antibody production and secretion in CHO cells

阐明 CHO 细胞中抗体产生和分泌的重要信号通路

• 批准号: 2467836
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2467836
• 关键词: Elucidating; signalling; pathways; important; antibody

Constitutive secretion is a fundamental cellular process required for the delivery of newlysynthesised proteins and lipids to the cell surface as well as the exocytosis of physiologicallyimportant molecules such as antibodies, cytokines and extracellular matrix proteins. This pathwayis conserved in all cell types and underpins biomanufacturing in CHO cells so has significantfinancial importance. Constitutive secretion has been extensively studied over the last 40 years sowe have a good understanding of the molecular machinery that acts on this pathway. However, itis not well understood how this machinery is coordinated and regulated by extracellular signals.The overarching aims of this project are to: 1) identify which extracellular signals regulate antibodyproduction and secretion; 2) determine how these signalling pathways change during antibodyproduction in CHO cells and 3) manipulate these pathways to enhance antibody production andperformance of CHO cells. This project will provide training in advanced mass-spectrometry andstate of the art, high-throughput, automated systems for cell culture and phenotypic analysis atGSK.This project is a collaboration between the University of Sheffield and GSK BiopharmaceuticalProcess Research, Stevenage so the student will spend a significant period of time based with thecompany. Candidates should have a strong academic degree in biological sciences. Thesuccessful candidate will be highly motivated, work effectively in a team setting and be interestedin cell biology, signalling and biomanufacturing. This studentship is available starting October 2020and is a 4 year BBSRC-funded Industrial CASE PhD studentship. Applicant eligibility criteria for thestudentship can be found at www.bbsrc.ac.uk/documents/studentship-eligibility-pdf/ and you mustidentify that you fit these criteria prior to application.

组成性分泌是将新合成的蛋白质和脂质递送到细胞表面以及将生理上重要的分子（如抗体、细胞因子和细胞外基质蛋白）胞吐所需的基本细胞过程。该途径在所有细胞类型中都是保守的，并支持CHO细胞中的生物制造，因此具有重要的经济意义。组成性分泌在过去的40年里得到了广泛的研究，因此我们对作用于这一途径的分子机制有了很好的理解。本研究的主要目的是：1）确定哪些细胞外信号调节抗体的产生和分泌; 2）确定这些信号通路在CHO细胞中抗体产生过程中的变化; 3）操纵这些信号通路以增强CHO细胞的抗体产生和性能。该项目将提供先进的质谱和最先进的，高通量的，自动化系统的细胞培养和表型分析在GSK的培训。该项目是谢菲尔德大学和GSK生物制药过程研究，斯蒂夫尼奇之间的合作，所以学生将花费大量的时间与公司为基础。候选人应在生物科学方面具有很强的学术学位。优秀的候选人将具有高度的积极性，在团队环境中有效地工作，并对细胞生物学，信号传导和生物制造感兴趣。该奖学金从2020年10月开始提供，是一个为期4年的BBSRC资助的工业案例博士生奖学金。申请奖学金的资格标准可以在www.bbsrc.ac.uk/documents/studentship-eligibility-pdf/上找到，你必须在申请前确定你符合这些标准。