Elucidating the signalling pathways important for antibody production and secretion in CHO cells

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Constitutive secretion is a fundamental cellular process required for the delivery of newlysynthesised proteins and lipids to the cell surface as well as the exocytosis of physiologicallyimportant molecules such as antibodies, cytokines and extracellular matrix proteins. This pathwayis conserved in all cell types and underpins biomanufacturing in CHO cells so has significantfinancial importance. Constitutive secretion has been extensively studied over the last 40 years sowe have a good understanding of the molecular machinery that acts on this pathway. However, itis not well understood how this machinery is coordinated and regulated by extracellular signals.The overarching aims of this project are to: 1) identify which extracellular signals regulate antibodyproduction and secretion; 2) determine how these signalling pathways change during antibodyproduction in CHO cells and 3) manipulate these pathways to enhance antibody production andperformance of CHO cells. This project will provide training in advanced mass-spectrometry andstate of the art, high-throughput, automated systems for cell culture and phenotypic analysis atGSK.This project is a collaboration between the University of Sheffield and GSK BiopharmaceuticalProcess Research, Stevenage so the student will spend a significant period of time based with thecompany. Candidates should have a strong academic degree in biological sciences. Thesuccessful candidate will be highly motivated, work effectively in a team setting and be interestedin cell biology, signalling and biomanufacturing. This studentship is available starting October 2020and is a 4 year BBSRC-funded Industrial CASE PhD studentship. Applicant eligibility criteria for thestudentship can be found at www.bbsrc.ac.uk/documents/studentship-eligibility-pdf/ and you mustidentify that you fit these criteria prior to application.