权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Osteoblast Response to Amorphous Calcium Phosphate

成骨细胞对无定形磷酸钙的反应

基本信息

批准号：
6884823
负责人：
BRIAN J LOVE
金额：
$ 7.19万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-05-01 至 2007-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6884823
关键词：
bioengineering /biomedical engineering biomaterial development /preparation biomaterial interface interaction bone regeneration calcium phosphate laboratory rat medical implant science oral facial restoration oral facial restoration material osteoblasts polymers tissue /cell culture tissue engineering tissue support frame

项目摘要

DESCRIPTION (provided by applicant): The broad, long-term objective is to evaluate how osteoblast response is mediated by nutrient transport provided by dissolving amorphous calcium phosphate (ACP) particulates. We hypothesize that the attraction of ACP lies in its higher dissolution rate, and its ready interaction with osteoblasts leading to more rapid mineralization. We propose that ACP mineral immobilized membranes could be the first step in creating viable scaffolds for structural repair and regeneration. The proposed effort is distributed among three linked Aims. Aim 1 will perform targeted cell culture studies of stabilized forms of ACP both separated by neighboring osteoblasts through a membrane in the Transwell plate and by direct contact experiments under cell culture conditions between cells and ACP. We will evaluate the release rate of target ions in solution and track cell viability and function with incubation time in growth media deprived of calcium phosphate. Protein expression will be evaluated by Northern blot and other gel and assay techniques. Aim 2 will construct model porous scaffolds incorporating and immobilizing stabilized ACP within them by a photopolymerizable pre-polymer dispersion. Aim 3 will evaluate osteoblast cell response to the immobilized ACP composites from cell culture using Transwell plates to compare cell viability and function in this different mode. Similar assays are planned in Aim 3 as in Aim 1 with the distinction that other controls will be required to separate the matrix effect from the mineral effect. Thus Aim 3 leverages off of the experience gained in cell culture during Aim 1 with the ability to fabricate mineral immobilized membrane matrices in Aim 2. At the end of the project, we will have compiled strong evidence linking ACP solubility, neighboring osteoblast viability, and tissue growth and proliferation. We believe that these foundation elements will provide the basis for a successful R-01 proposal concept to be submitted as this project is completed.

描述(由申请人提供)：广泛的、长期的目标是评估成骨细胞的反应是如何通过溶解无定形磷酸钙(ACP)颗粒所提供的营养运输来调节的。我们推测，ACP的吸引力在于其较高的溶解速度，以及它与成骨细胞的容易相互作用导致更快的矿化。我们认为，ACP矿物固定化膜可能是创造可用于结构修复和再生的可行支架的第一步。拟议的努力分布在三个相互关联的目标上。目的1将对稳定形式的ACP进行有针对性的细胞培养研究，包括相邻成骨细胞通过Transwell板中的膜分离，以及细胞与ACP在细胞培养条件下的直接接触实验。我们将评估目标离子在溶液中的释放速率，并跟踪细胞在不含钙磷的生长介质中孵育时间的活性和功能。蛋白质的表达将通过Northern印迹和其他凝胶和分析技术进行评估。目的2将通过可光聚合的预聚体分散体在其中加入并固定稳定的ACP，以构建模型多孔支架。目的3将使用Transwell平板评估成骨细胞对来自细胞培养的固定化ACP复合材料的反应，以比较在不同模式下的细胞存活率和功能。目标3与目标1计划进行类似的分析，区别在于需要其他控制措施将基质效应与矿物效应分开。因此，Aim 3利用了在Aim 1中获得的细胞培养经验，并在Aim 2中构建了矿物固定膜基质。在项目结束时，我们将汇编强有力的证据，将ACP的溶解度、邻近的成骨细胞活性以及组织生长和增殖联系起来。我们相信，这些基础要素将为在该项目完成时提交成功的R-01提案概念提供基础。