Seasonal Adaptation of a Circadian Clock

昼夜节律钟的季节适应

基本信息

  • 批准号:
    6925193
  • 负责人:
  • 金额:
    $ 34.04万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2001
  • 资助国家:
    美国
  • 起止时间:
    2001-07-15 至 2009-02-28
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): The overall goal of this proposal is to understand how a circadian pacemaker accommodates new phase alignments in response to seasonal changes in ambient temperature and day length (photoperiod). A major foundation for the proposed specific aims is based on our prior work showing that the thermosensitive and photosensitive splicing of the 3'-terminal intron from the D. melanogaster period (per) RNA (termed dmpi8; D. melanogaster dper intron 8) acts as a multi-modal "seasonal sensor" conveying calendar information. High splicing efficiency of dmpi8 on seasonally cold days (i.e., low temperatures and short photoperiods) advances the timing of 'evening' activity, endowing flies with the ability to manifest daytime activity despite the early onset of dusk. Conversely, low splicing efficiency of dmpi8 on warm days ensures that flies minimize activity during the hot midday sun and are mainly nocturnal, lowering the risks associated with desiccation. We also showed that the norpA (no receptor potential A) gene encoding phospholipase C (PLCbeta), modulates the temperature and clock control of dmpi8 splicing efficiency. These findings identified a novel non-photic role for norpA. In this application we propose to determine how temperature, light, clock factors and NORPA co-regulate the splicing efficiency of dmpi8, and the contribution of this molecular event to seasonal adaptation in rhythmic behavior. Major objectives are to identify cis-acting elements that mediate thermosensitivity of dmpi8 splicing and investigate the role of the PLC/phosphatidylinositol signaling cascade. Moreover, we will analyze the clockworks and behavioral rhythms in natural populations of several different Drosophila species isolated from a wide range of latitudes. Preliminary findings suggest that per 3'-terminal introns are targets for natural selection in the adaptation of circadian clocks to geographical variations in seasonal climates. To accomplish these goals we propose to take multifacted experimental strategies including biochemical, tissue culture and whole animal approaches. Our studies will add insights into seasonal adaptation of clock function and reveal how norpA participates in calibrating molecular and behavioral thermoresponses in D. melanogaster.
描述(由申请人提供):本提案的总体目标是了解昼夜节律起搏器如何适应环境温度和日长(光周期)的季节性变化而适应新的相位对准。提出的特定目标的一个主要基础是基于我们先前的工作,即从黑腹果蝇时期(PER)RNA(称为dmpi8;D.Blackogaster dper内含子8)的3‘端内含子的温度和光敏剪接起到传达日历信息的多模式“季节传感器”的作用。在季节性寒冷的日子(即低温和短光周期),dmpi8的高剪接效率提前了‘晚上’活动的时间,使苍蝇能够在黄昏很早的时候表现出白天的活动。相反,在温暖的日子里,dmpi8的低剪接效率确保了苍蝇在正午炎热的阳光下尽量减少活动,而且主要是夜间活动,从而降低了与干燥相关的风险。我们还发现编码磷脂酶C(PLCbeta)的NORPA(无受体潜势A)基因调节温度和时钟控制dmpi8的剪接效率。这些发现确定了Norpa的一个新的非光作用。在这个应用中,我们建议确定温度、光、时钟因素和Norpa如何共同调节dmpi8的剪接效率,以及这个分子事件对节律行为的季节性适应的贡献。主要目的是确定介导dmpi8剪接温度敏感性的顺式作用元件,并研究PLC/磷脂酰肌醇信号级联的作用。此外,我们还将分析来自不同纬度的几个不同果蝇物种的自然种群的时钟和行为节律。初步研究结果表明,在生物钟适应季节气候地理变化的过程中,Per 3‘末端内含子是自然选择的目标。为了实现这些目标,我们建议采取多因素实验策略,包括生化、组织培养和全动物方法。我们的研究将增加对时钟功能季节性适应的洞察,并揭示Norpa如何参与校准黑腹毛虫的分子和行为热反应。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

ISAAC EDERY其他文献

ISAAC EDERY的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('ISAAC EDERY', 18)}}的其他基金

Mechanisms Underlying Seasonal Adaptation of Daily Behaviors
日常行为季节性适应的机制
  • 批准号:
    10183348
  • 财政年份:
    2018
  • 资助金额:
    $ 34.04万
  • 项目类别:
Mechanisms Underlying Seasonal Adaptation of Daily Behaviors
日常行为季节性适应的机制
  • 批准号:
    10406158
  • 财政年份:
    2018
  • 资助金额:
    $ 34.04万
  • 项目类别:
Mechanisms Underlying Seasonal Adaptation of Daily Behaviors
日常行为季节性适应的机制
  • 批准号:
    9920226
  • 财政年份:
    2018
  • 资助金额:
    $ 34.04万
  • 项目类别:
Clock Mechanism Underlying Drosophila Rhythmic Behavior
果蝇节律行为的时钟机制
  • 批准号:
    9336138
  • 财政年份:
    2016
  • 资助金额:
    $ 34.04万
  • 项目类别:
Clock Mechanism Underlying Drosophila Rhythmic Behavior
果蝇节律行为的时钟机制
  • 批准号:
    9012966
  • 财政年份:
    2015
  • 资助金额:
    $ 34.04万
  • 项目类别:
Seasonal Adaptation of a Circadian Clock
昼夜节律钟的季节适应
  • 批准号:
    6540508
  • 财政年份:
    2001
  • 资助金额:
    $ 34.04万
  • 项目类别:
Seasonal Adaptation of a Circadian Clock
昼夜节律钟的季节适应
  • 批准号:
    8288134
  • 财政年份:
    2001
  • 资助金额:
    $ 34.04万
  • 项目类别:
Seasonal Adaptation of a Circadian Clock
昼夜节律钟的季节适应
  • 批准号:
    7888920
  • 财政年份:
    2001
  • 资助金额:
    $ 34.04万
  • 项目类别:
Seasonal Adaptation of a Circadian Clock
昼夜节律钟的季节适应
  • 批准号:
    8016560
  • 财政年份:
    2001
  • 资助金额:
    $ 34.04万
  • 项目类别:
Seasonal Adaptation of a Circadian Clock
昼夜节律钟的季节适应
  • 批准号:
    8473922
  • 财政年份:
    2001
  • 资助金额:
    $ 34.04万
  • 项目类别:

相似海外基金

Mechanisms of messenger RNA splicing and RNA processing regulation
信使RNA剪接和RNA加工调控机制
  • 批准号:
    10623834
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
Analysis on how RNA splicing factors change global gene expression patterns and regulate male fertility.
分析RNA剪接因子如何改变全局基因表达模式并调节男性生育能力。
  • 批准号:
    2882792
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
    Studentship
Collaborative Research: Connecting the sequence logic of RNA splicing to nuclear localization
合作研究:将 RNA 剪接的序列逻辑与核定位联系起来
  • 批准号:
    2246530
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
    Standard Grant
Collaborative Research: Connecting the sequence logic of RNA splicing to nuclear localization
合作研究:将 RNA 剪接的序列逻辑与核定位联系起来
  • 批准号:
    2246531
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
    Standard Grant
Aberrant RNA splicing in sporadic inclusion body myositis
散发性包涵体肌炎中的异常RNA剪接
  • 批准号:
    23K18260
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Synthetic introns for selective targeting of RNA splicing factor-mutant leukemia
用于选择性靶向RNA剪接因子突变型白血病的合成内含子
  • 批准号:
    10722782
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
Cancer immune therapeutics targeting aberrant RNA splicing products
针对异常 RNA 剪接产物的癌症免疫疗法
  • 批准号:
    23H02688
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
RNA splicing regulation during alcohol withdrawal
酒精戒断过程中的 RNA 剪接调节
  • 批准号:
    10785159
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
Targeting Dysregulated RNA Splicing in Neurodegenerative Diseases
靶向神经退行性疾病中失调的 RNA 剪接
  • 批准号:
    10729566
  • 财政年份:
    2023
  • 资助金额:
    $ 34.04万
  • 项目类别:
Function, composition, and mechanism of RNA splicing factories in cardiomyopathy
RNA剪接工厂在心肌病中的功能、组成和机制
  • 批准号:
    10583011
  • 财政年份:
    2022
  • 资助金额:
    $ 34.04万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了