Fingerprinting of (cancer) cells using integrated nanophotonics and plasmonic nanopore sensing

使用集成纳米光子学和等离子体纳米孔传感对（癌症）细胞进行指纹识别

• 批准号: 2482369
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2482369
• 关键词: Fingerprinting; cancer; cells; using; integrated

The technology we envisage will allow measuring the biochemical fingerprint of 100s of intercellular targets at the single cell level. From the sequencing of cancerous cells extracted from tumours, it is also known that the heterogeneity of cells is not only seen in their DNA sequence but also their contents. The combination of Nanophotonics and Nanopore based sensing as proposed here will be able to detect hundreds of targets simultaneously extracted from single cells. The main advantage of the combination of photonic sensing and single molecule based nanopore technique is the ultra-low sample amounts of femtomoles - orders of magnitude below the usual requirements of DNA microarray. In combination with microfluidics, we will detect as few as 1000 copies of a target molecule in a cell. The combination of label free optical scattering (DLS and SERS) with nanopores offer unprecedented increases information. The nanophotonic-nanopore-microfluidic system to be developed aims to detect a variety of small molecules and DNA/RNA fragments. This exploratory investigation will try to understand the different signatures that can be extracted from lysed cells that are cancerous or not, and the range of heterogeneity. The ability to fingerprint cell by cell is crucial.

我们设想的技术将允许在单细胞水平上测量100个细胞间目标的生化指纹。从从肿瘤中提取的癌细胞的测序中，我们也知道细胞的异质性不仅体现在它们的DNA序列上，而且体现在它们的内容物上。本文提出的纳米光子学和纳米孔传感的结合将能够同时检测从单个细胞中提取的数百个目标。光子传感与单分子纳米孔技术相结合的主要优点是飞摩尔的样本量极低，比DNA微阵列的通常要求低几个数量级。结合微流体，我们将检测到细胞中目标分子的1000个拷贝。无标签光散射（DLS和SERS）与纳米孔的结合提供了前所未有的增加信息。待开发的纳米光子-纳米孔微流控系统旨在检测各种小分子和DNA/RNA片段。这项探索性研究将试图了解从裂解细胞中提取的不同特征，这些特征可以是癌变的或非癌变的，以及异质性的范围。一个细胞一个细胞地识别指纹的能力至关重要。