CORE B--BLOOD CELL SEPARATION AND MHC TYPING

Core B是血细胞分离和MHC分型的Core。 该设施包括两个部分：每个部分都有专用的通风橱、培养箱和液氮以及-80摄氏度的冷冻机。 第一部分执行从全血到PBMC、血浆和血清样本的所有样本处理以及这些样本的冷冻。 当项目的个别要求需要时，核心B的第一部分还分离了核心B的B、T、NK、NKT，进行MHC等位基因分型，包括补体分型和B细胞的EBV转化，以生成、储存和维护B淋巴母细胞系。 基本分型方案包括通过PCR和HLA-A、B、C、DR B（根据需要包括高分辨率）、DQA、DQ B、DPA和DPB寡核苷酸探针杂交进行DNA分型，以及BF、C2、C4 A和C4 B的蛋白分型。 核心B的第2部分还根据需要进行ABO血型分型。

Core B is a blood cell separation and MHC typing Core. The facility comprises two parts: each has devoted hoods, incubators and liquid nitrogen and -80 degrees C freezers. The first part performs all sample processing from whole blood to PBMC, plasma and serum samples and the freezing of these samples. When required for individual requirements of the Projects, the first part of Core B also separates B, T, NK, NKT, of Core B carries out MHC allele typing, including complement typing, and EBV transformation of B cells to generate, bank and maintain B lymphoblastoid cell lines. The basic typing regimen includes DNA typing by PCR and hybridization with oligonucleotide probes of HLA-A, B, C, DRB (including high resolution as needed), DQA, DQB, DPA and DPB and protein typing for BF, C2, C4A and C4B. Part 2 of Core B also performs ABO blood group typing as needed.