Exploring the Scope and Mechanism of Immune-Suppression by a Novel P-Rex1 Interacting Protein

探索新型 P-Rex1 相互作用蛋白免疫抑制的范围和机制

• 批准号: 2483427
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2483427
• 关键词: Exploring; Scope; Mechanism; Immune; Suppression

Initially, the student will test the ability of PIP-deficient and PIP/Prex1 deficient neutrophils to migrate by chemotaxis and chemokinesis. The student will use isolated neutrophils from wild-type, PIP-deficient and PIP/Prex1 deficient mice to evaluate receptor trafficking and the principal signalling pathways and effector responses, employing various techniques such as imaging, western blotting and flow cytometry. S/he will use affinity reagents and mass spectrometry to identify molecular targets of PIP in neutrophils. S/he will also assess neutrophil recruitment to inflamed and infected tissues and the capacity to kill pathogens, using methods such as lavages, histology and intravital microscopy. Thus, s/he will become an expert in signalling, trafficking, leukocyte biology and innate immunity. Moreover, his/her findings may have significant impact on future treatment strategies in inflammatory diseases that are exacerbated by excessively recruited and activated neutrophils

最初，学生将测试PIP缺陷和PIP/Prex 1缺陷中性粒细胞通过趋化性和趋化运动迁移的能力。学生将使用从野生型，PIP缺陷和PIP/Prex 1缺陷小鼠分离的中性粒细胞来评估受体运输和主要信号传导途径和效应器反应，采用各种技术，如成像，蛋白质印迹和流式细胞术。她/他将使用亲和试剂和质谱法来识别中性粒细胞中PIP的分子靶点。他/她还将评估中性粒细胞募集到发炎和感染组织以及杀死病原体的能力，使用灌洗，组织学和活体显微镜等方法。因此，她/他将成为信号，贩运，白细胞生物学和先天免疫方面的专家。此外，他/她的研究结果可能对过度募集和活化中性粒细胞加剧的炎症性疾病的未来治疗策略产生重大影响。

项目成果

Investigating the immunosuppressive functions of the GPCR adapter protein Norbin

研究 GPCR 接头蛋白 Norbin 的免疫抑制功能

• DOI: 10.17863/cam.104740
• 发表时间: 2023
• 作者: Chetwynd S