The role of CEACAM receptors in streptococcal infections

CEACAM 受体在链球菌感染中的作用

• 批准号: 2496995
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2496995
• 关键词: role; CEACAM; receptors; streptococcal; infections

1. To characterise the molecular mechanism of the R28IgI3 adhesin and CEACAM1 receptor interaction2. To assess whether B and R28 induce CEACAM1 signalling3. To define the function of CEACAM engagement with GBS and GAS in the context of epithelial cellsCarcinoembryonic antigen-related adhesion molecules (CEACAM) receptors are expressed on wide range of cells including epithelial cells and leukocytes. They modulate immune responses through forming homophilic and heterophilic interactions, and serve as docking sites for several human pathogens. We previously discovered that human streptococcal pathogens can express adhesins that bind human CEACAM receptors with high-affinity and high-specificity. This project will investigate how human streptococcal pathogens utilise CEACAM receptors to colonise mucosal surfaces and to cause invasive infections. We will combine various techniques including biochemical techniques and cellular infection assays. To complement this approach, we will utilise structural biology approaches to analyse host-pathogen interactions in molecular detail. As Streptococcal pathogens remain a significant and no vaccines currently exist, research into their virulence is of importance for development of novel therapeutic strategies.

1.阐明R28 IgI 3粘附素与CEACAM 1受体相互作用的分子机制2.评估B和R28是否诱导CEACAM 1信号传导3。为了明确CEACAM与GBS和GAS在上皮细胞中的作用，癌胚抗原相关粘附分子（CEACAM）受体在包括上皮细胞和白细胞在内的广泛细胞上表达。它们通过形成嗜同性和嗜异性相互作用来调节免疫反应，并作为几种人类病原体的对接位点。我们以前发现，人类链球菌病原体可以表达粘附素，以高亲和力和高特异性结合人类CEACAM受体。该项目将研究人类链球菌病原体如何利用CEACAM受体定植在粘膜表面并引起侵袭性感染。我们将结合联合收割机各种技术，包括生物化学技术和细胞感染测定。为了补充这种方法，我们将利用结构生物学方法来分析宿主-病原体相互作用的分子细节。由于链球菌病原体仍然是一个重要的，目前还没有疫苗存在，研究其毒力是重要的新的治疗策略的发展。