Nanopore Sensors for Multiplexed Analysis of DNA in Clinically Relevant Samples

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Nanopores have become a versatile sensing technique for the single molecule analysis of DNA, RNA and proteins. Single molecules are detected by measuring changes in ionic current flow as they pass through a nanometre scale pore separating two chambers containing salt solution. However, to advance past an initial proof of principal stage for nanopore sensing, several key areas need to be improved. This project will investigate the development of a robust nanopore sensing assay for multiplexed detection of diverse biomolecules in complex clinically relevant samples. The project will require innovation on several fronts, including redesigning and optimizing the sensing system for sensing in complex solutions, investigating benefits of functionalizing the nanopores for sensor performance, and proof of concept tests detecting and quantifying multiple biomarkers concurrently in clinical samples. The project will investigate and develop sensing protocols for counting biomarkers and genes. As the nanopore sensing technique allows for the general counting and sizing of molecules binding to DNA, the project will also aim to push the limits to use the sensors for analysing the mass of bound proteins down to sub kDa. The latter is relevant to identify protein modification and small ligand binding events and should allow for unique insights into the workings of living organisms.