Microbiologically influenced corrosion of maritime platforms

微生物影响的海上平台腐蚀

• 批准号: 2517128
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2517128
• 关键词: Microbiologically; influenced; corrosion; maritime; platforms

A considerable amount of research has been performed to determine the effects of destructive biofilms formed on metallic systems, most of which have been in the oil and gas, and energy industries. Although in more recent years there has been an increased interest in an effective means of detecting, monitoring, and preventing corrosion an understanding of the microbial community consortium in association with MIC is still lacking. Consequently, the identities of the bacterial consortia involved in the reactions are not fully known, which can impede the formulation of a targeted mitigation strategy for such issues. To be able to contribute to the future of anti-corrosion strategies of maritime platforms this Ph.D. programme will replicate the polymicrobial community identified at field sites, using culture-independent techniques, to develop an understanding of the bacterial consortia which could form problematic biofilms on maritime platforms for BAE systems. The proposed work will be separated into three stages and the objectives associated with each of these are as follows: Characterise corrosive biofilms found at selected docks, used by BAE systems for maritime platform storage and maintenance, to identify and isolate key microorganisms involved in biofilm activities Construct synthetic laboratory consortia to replicate the different communities identified from maritime platforms Use the synthetic community to define the roles of different bacteria in those biofilms enabling an understanding of how microbiologically influenced corrosion (MIC) associated with them might be controlled Techniques for studying individual microorganisms in the laboratory are well established and advancements in technologies now enable culture-independent characterisation of complex polymicrobial systems at the DNA level, removing the bias of which organisms are easiest to grow in isolation in the laboratory. More recently, progress has been made in several areas of microbiology by combining these two techniques, to construct artificial consortia in the laboratory that can be used to probe the roles of the individual members and to observe how the composition of the consortium changes with time and environmental stresses. Biofilm samples will be taken from sites of interest and total DNA will be prepared and used for barcoded 16S rRNA gene-specific PCR followed by next generation DNA sequencing to determine the bacterial species composition in the biofilms. This information will be used to inform enrichment experiments to isolate representatives of key groups of microorganisms from the biofilm samples. Wherever possible the isolates will correspond to the major players in the original biofilms, though even where this is not possible (because dominant organisms in complex polymicrobial communities in the environment often do not grow easily in laboratory media), representatives of the key functional groups will be obtained. This will allow subsequent experiments with artificially constructed communities of microorganisms in the laboratory, which will allow the effects of various corrosion promoting and corrosion inhibiting microorganisms to be studied in various combinations in a flow cell system that will allow observations to be made by electrochemical methods. The results are expected to inform future anti-corrosion strategies, including use of non-biocidal agents to modify biofilm behaviour and development of functional coatings containing key anticorrosion/ antifouling microorganisms.

人们进行了大量研究，以确定金属系统上形成的破坏性生物膜的影响，其中大部分是在石油和天然气以及能源行业。尽管最近几年人们对检测、监测和预防腐蚀的有效手段越来越感兴趣，但对与MIC有关的微生物群落联盟的了解仍然不足。因此，参与反应的细菌联合体的身份并不完全清楚，这可能会阻碍针对此类问题制定有针对性的缓解战略。为了能够为海洋平台未来的防腐战略做出贡献，这一博士项目将利用独立于培养的技术，复制在现场确定的多微生物群落，以加深对细菌群落的了解，这些细菌可能在海洋平台上形成BAE系统的问题生物膜。拟议的工作将分为三个阶段，与每个阶段相关的目标如下：表征在选定码头发现的腐蚀性生物膜，由BAE系统用于海洋平台存储和维护，以识别和隔离参与生物膜活动的关键微生物构建合成实验室联合体，以复制从海洋平台识别的不同群落使用合成群落来定义不同细菌在这些生物膜中的作用，从而能够理解与它们相关的微生物影响腐蚀(MIC)可能如何被控制，用于在实验室研究单个微生物的技术已经确立，并且技术的进步现在能够在DNA水平上对复杂的多菌系统进行独立于培养的表征，消除了哪种生物最容易在实验室中隔离生长的偏见。最近，通过将这两种技术结合起来，在微生物学的几个领域取得了进展，在实验室建立了人工联合体，可以用来探索个别成员的作用，并观察联合体的组成如何随着时间和环境压力的变化而变化。将从感兴趣的地点采集生物膜样本，并制备总DNA，用于条形码16S rRNA基因特异性聚合酶链式反应，然后进行下一代DNA测序，以确定生物膜中的细菌种类组成。这些信息将用于浓缩实验，以便从生物膜样品中分离出关键微生物组的代表。只要有可能，分离物将与原始生物膜中的主要角色相对应，尽管即使在不可能的情况下(因为环境中复杂的多菌群落中的优势生物通常不容易在实验室介质中生长)，也将获得关键功能群的代表。这将允许在实验室中进行后续的人工构建微生物群落的实验，这将允许在流动电池系统中以各种组合来研究各种促进腐蚀和抑制腐蚀的微生物的效果，这将允许通过电化学方法进行观察。预计这些结果将为未来的防腐战略提供参考，包括使用非杀生剂来改变生物膜的行为，以及开发含有关键防腐/防污微生物的功能涂料。