In Vitro High Throughput Screening Assay for MKP-3(RMI)

MKP-3(RMI) 体外高通量筛选试验

• 批准号: 7058166
• 负责人: JOHN S. LAZO
• 金额: $ 0.45万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2006-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7058166
• 关键词: biological signal transduction; biotechnology; combinatorial chemistry; drug screening /evaluation; high throughput technology; mitogen activated protein kinase; phosphatase inhibitor; protein tyrosine phosphatase; small molecule; technology /technique development

DESCRIPTION (provided by applicant): Mitogen activated protein kinase phosphatase-3 (MKP-3) is a dual specificity phosphatase that has a central role regulating intracellular signal transduction by controlling the phosphorylation status of mitogen activated protein kinases. There is considerable evidence indicating MKP-3 could be a potential molecular target for several human diseases. Nonetheless, no potent or selective small molecule inhibitors of this protein phosphatase have been identified. This proposal will provide sufficient reagents to permit a high throughput screening of 100,000 compounds

说明书(申请人提供)：丝裂原活化蛋白激酶磷酸酶-3(MKP-3)是一种双特异性磷酸酶，通过控制丝裂原活化蛋白激酶的磷酸化状态来调节细胞内的信号转导。有相当多的证据表明，MKP-3可能是几种人类疾病的潜在分子靶点。尽管如此，目前还没有发现这种蛋白磷酸酶的有效或选择性小分子抑制剂。这项提议将提供足够的试剂来高通量筛选100,000种化合物