Olfactory Map Formation In Zebrafish

斑马鱼嗅觉图的形成

• 批准号: 7055051
• 负责人: TODD P FERREIRA
• 金额: $ 2.96万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7055051
• 关键词: alternatives to animals in research; biotechnology; chemoreceptors; confocal scanning microscopy; developmental neurobiology; electrophysiology; gene expression profiling; genetically modified animals; green fluorescent proteins; molecular biology; neuronal guidance; neurons; olfactions; olfactory lobe; polymerase chain reaction; predoctoral investigator; receptor expression; zebrafish

DESCRIPTION (provided by applicant): In the olfactory system, olfactory receptor neurons (ORNs) distributed widely in the sensory epithelium converge with great precision on specific points in the brain to form an invariant map of odorant representation. The molecular mechanism that governs this phenomenon is not fully understood. Experiments presented in this application will address this question by using the zebrafish to develop a transgenic system which will allow in vivo observation of olfactory development. Subsets of olfactory receptor neurons will be tagged with GFP expression in an odorant receptor-specific manner. The development of these tagged neurons will be characterized in stable transgenic zebrafish embryos and the overall development of the zebrafish olfactory system will be elucidated. Through genetic manipulation and in vivo imaging, I will determine the nature of the olfactory sensory map in the zebrafish, the role of the olfactory receptor in the targeting of ORNs, and the possible role of pioneer neurons in establishing the spatial map. Elucidating the molecular mechanisms underlying olfactory map formation in zebrafish will pave the way for detailed genetic analysis of axon pathfinding and coding in the olfactory system.

描述（由申请人提供）：在嗅觉系统中，嗅觉受体神经元（ORNS）广泛分布在感觉上皮中，在大脑中的特定点上非常精确地收敛，形成了odortant的不变图。控制这种现象的分子机制尚未完全理解。本应用程序中提出的实验将通过使用斑马鱼开发转基因系统来解决这个问题，该系统将允许体内观察嗅觉开发。嗅觉受体神经元的子集将以气味受体特异性方式标记为GFP表达。这些标记的神经元的发展将以稳定的转基因斑马鱼胚胎为特征，斑马鱼嗅觉系统的整体发育将被阐明。通过遗传操纵和体内成像，我将确定斑马鱼中嗅觉感觉图的性质，嗅觉受体在ORN靶向中的作用，以及先锋神经元在建立空间图中的可能作用。阐明斑马鱼中嗅觉图的形成的分子机制将为嗅觉系统中的轴突探路和编码详细遗传分析铺平道路。