The genetics of reproductive isolation in Leavenworthia

莱文沃斯属生殖隔离的遗传学

• 批准号: 6820637
• 负责人: Vanessa Anne Koelling
• 金额: $ 4.1万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-11-15 至 2007-11-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6820637
• 关键词: genetics; reproductive; isolation; Leavenworthia

18. GOALS FOR FELLOWSHIP TRAINING AND CAREER NAME (Last, first, middle initial) Koelling, Vanessa, A. INSTITUTION MENTOR College, Portland, OR Dr. Keith Karoly SUPERVISOR/EMPLOYER INSTITUTION/COMPANY Reed College Dr. Keith Karoly As an NIH pre-doctoral fellow and Ph.D. candidate at the University of Georgia, my primary goal will be to study the ecological and molecular genetics of speciation in the plant genus Leavenworthia (Brassicaceae). Undertaking this proposed research will allow me to meet other complementary goals during my fellowship training as well. These complementary goals include learning new field and laboratory techniques, and gaining more experience in the use of those techniques. In particular, I plan to be more extensively trained in molecular genetic techniques for the examination of gene structure and function, chromosome structure and function, and genome evolution as a whole. Molecular genetic techniques will aid me in completing more comprehensi,m studies of the genetics of speciation than could be done using a purely ecological genetics approach. After I receive my doctoral degree from the University of Georgia, I intend to continue studying the genetics of speciation while teaching evolutionary genetics courses at the college or university level. Ultimately, my fellowship training and graduate education will play a pivotal role in my intellectual development and career growth. -'1i'Jl _'l'ii 19. NAME AND DEGREE(S) Rodney Mauricio, Ph.D. 20. POSITION/RANKAssistant Professor of Genetics 21. RESEARCH INTERESTS/AREAS The genetics and evolution of complex traits; population genetics, ecological genetics; evolutionary genetics il=_1_'11i|'_:I" :{,li{,lql', 22. DESCRIPTION (Do not exceed space provided) Understanding how species form is one of the fundamental unanswered questions of evolutionary genetics. And it is critical in understanding genome evolution. Answering this question requires knowledge of the genetic basis of reproductive isolation between species. Specific questions are: (1) what evolutionary processes are responsible for reproductive barriers between species?; (2) what are the frequency of certain barriers to reproduction between species'?.; (3) how many loci contribute to the quantitative traits associated with reproductive isolation between species?; and (4) what genes control reproductive isolation between species and how do they function? To begin to answer these questions, the ecological and genetic basis of reproductive isolation between two species in the plant genus Leavenworthia (L. alabamica and L. crassa) will be determined. Reciprocal transplant experiments will determine whether or not ecogeographic isolation is present. Pollinator observations will be conducted to see if pollinator preferences differ between species, which would be a form of ethological isolation. Leavenworthia alabamica and L. crassa hybridize in nature, and thus hybrid viability will be examined in order to determine if any post-zygotic barriers to reproduction exist. Quantitative trait loci will be mapped to determine how many genetic loci are responsible for reproductive isolation. These QTL will be compared to the sequence of Arabidopsis thaliana as a clue to gone function. Finally, the genetics of any inbreeding depression observed in these species or their hybrids will be determined. PHS 416-1 (Rev. 12/98) Form Page 2 BB cc Individual NRSA Application Table of Contents ========================================Section End===========================================

18.奖学金培训目标和职业名称（姓，名，中间名）Koelling，Vanessa，A。机构Mentor College，波特兰，或Dr.基思Karoly主管/雇主机构/公司Reed College Dr.基思Karoly作为NIH博士前研究员和博士作为格鲁吉亚大学的一名候选人，我的主要目标将是研究植物属Leavenacroia（菊科）物种形成的生态和分子遗传学。从事这项拟议中的研究将使我能够在我的奖学金培训期间实现其他补充目标。这些互补的目标包括学习新的领域和实验室技术，并获得更多的经验，在使用这些技术。特别是，我计划更广泛地接受分子遗传学技术的培训，以检查基因结构和功能，染色体结构和功能，以及整个基因组的进化。分子遗传学技术将帮助我完成比纯生态遗传学方法更全面的物种形成遗传学研究。在我从格鲁吉亚大学获得博士学位后，我打算继续研究物种形成的遗传学，同时在学院或大学教授进化遗传学课程。最终，我的奖学金培训和研究生教育将在我的智力发展和职业发展中发挥关键作用。19. baby baby baby姓名和学位Rodney Mauricio，Ph.D. 20.职位/职级遗传学助理教授21.研究兴趣/领域复杂性状的遗传学和进化;群体遗传学、生态遗传学;进化遗传学|'_：I”：{，li{，lql'，22.理解物种是如何形成的是进化遗传学的基本未回答的问题之一。这对理解基因组进化至关重要。要回答这个问题，需要了解物种间生殖隔离的遗传基础。具体的问题是：（1）什么样的进化过程是物种间生殖障碍的原因？(2)物种之间的某些繁殖障碍的频率是多少？(3)有多少基因座对与物种间生殖隔离相关的数量性状有贡献？（4）哪些基因控制着物种间的生殖隔离，它们是如何起作用的？为了开始回答这些问题，本文首先探讨了两种植物Leavenacrotia（L.）alabamica和L.将决定。相互移植实验将确定是否存在生态地理隔离。将进行传粉者观察，看看传粉者的偏好是否在物种之间存在差异，这将是一种行为学隔离的形式。Leavenacrotia alabamica和L. crassa在自然界中杂交，因此将检查杂种的生存力以确定是否存在任何合子后繁殖障碍。将绘制数量性状基因座，以确定有多少遗传基因座负责生殖隔离。将这些QTL与拟南芥的序列进行比较，作为研究gone功能的线索。最后，将确定在这些物种或其杂交种中观察到的任何近交衰退的遗传学。PHS 416-1（12/98修订版）表格第2页BB cc个人NRSA申请目录==