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The genetics of reproductive isolation in Leavenworthia

莱文沃斯属生殖隔离的遗传学

基本信息

批准号：
7525233
负责人：
Vanessa Anne Koelling
金额：
$ 2.19万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-03-18 至 2008-05-14
项目状态：
已结题

项目摘要

Understanding how species form is one of the fundamental unanswered questions of evolutionary genetics. And it is critical in understanding genome evolution. Answering this question requires knowledge of the genetic basis of reproductive isolation between species. Specific questions are: (1) what evolutionary processes are responsible for reproductive barriers between species?; (2) what are the frequency of certain barriers to reproduction between species'?.; (3) how many loci contribute to the quantitative traits associated with reproductive isolation between species?; and (4) what genes control reproductive isolation between species and how do they function? To begin to answer these questions, the ecological and genetic basis of reproductive isolation between two species in the plant genus Leavenworthia (L. alabamica and L. crassa) will be determined. Reciprocal transplant experiments will determine whether or not ecogeographic isolation is present. Pollinator observations will be conducted to see if pollinator preferences differ between species, which would be a form of ethological isolation. Leavenworthia alabamica and L. crassa hybridize in nature, and thus hybrid viability will be examined in order to determine if any post-zygotic barriers to reproduction exist. Quantitative trait loci will be mapped to determine how many genetic loci are responsible for reproductive isolation. These QTL will be compared to the sequence of Arabidopsis thaliana as a clue to gone function. Finally, the genetics of any inbreeding depression observed in these species or their hybrids will be determined.

了解物种是如何形成的是进化遗传学的一个基本未解问题。这对理解基因组进化至关重要。要回答这个问题，需要了解遗传学的知识。物种间生殖隔离的基础。具体问题有：（1）什么是进化过程物种之间的生殖障碍？(2)繁殖障碍的频率是多少物种之间的差异。(3)有多少基因座与生殖隔离相关的数量性状有关物种间的差异？（4）哪些基因控制着物种间的生殖隔离，它们是如何起作用的？到开始回答这些问题，两个物种之间生殖隔离的生态和遗传基础， Leavenacrotia（L.）alabamica和L.将决定。交互移植实验将确定是否存在生态地理隔离。将进行授粉观察，以了解传粉者的偏好因物种而异，这可能是一种行为学上的隔离。莱维内西亚 alabamica和L. crassa在自然界中杂交，因此将检查杂交体的活力以确定是否存在任何杂交体。合子后存在繁殖障碍。将绘制数量性状基因座，以确定有多少遗传基因座导致生殖隔离。这些QTL将与拟南芥的序列进行比较作为功能消失的线索最后，在这些物种中观察到的任何近亲繁殖衰退的遗传学或其将确定混合动力车。