Genetics of postdauer developmental timing in C elegans

描述（由申请人提供）：不同组织之间以及生物体与环境之间的细胞分裂和分化的协调时间是正常生长的关键方面。控制这些过程很重要，因为细胞分裂与生长控制信号无关是癌症的标志。线虫的异时基因控制着发育过程中细胞分裂和分化的相对时间。这些基因的突变导致一些细胞系要么省略要么重复其部分发育程序，导致各种异常组织。然而，如果幼虫在不利条件下通过另一种称为“dawer”的途径发育，许多这些基因的突变表型可以被抑制。这表明，一些先前表征的异慢性基因在产后发育中是必不可少的，这就提出了什么基因控制产后幼虫发育时间的问题。该项目的目的是识别和表征这些基因。具体目的1：开展基因筛选，分离发育时间缺陷突变体。专项目标2：对上述筛选中分离的基因进行遗传分析。特异性目标3：绘制和克隆在筛选中分离的突变体。

DESCRIPTION (provided by applicant): The coordinated timing of cell divisions and differentiation between different tissues and between the organism and the environment are critical aspects of normal growth. The control of these processes is important, as cell division irrespective of growth control signals is a hallmark of cancer. Heterochronic genes in C. elegans control the relative timing of cell divisions and differentiation during development. Mutations in these genes result in some cell lineages either omitting or reiterating parts of their developmental program, leading to a variety of abnormal tissues. However, the mutant phenotypes of many of these genes can be suppressed if larvae develop through an alternative pathway called "dauer", chosen in unfavorable conditions. This suggests that some previously characterized heterochronic genes are dispensable for postdauer development, raising the question of what genes control developmental timing in postdauer larvae. The objective of this project is to identify and characterize such genes. Specific Aim 1: To carry out genetic screens to isolate mutants defective in developmental timing postdauer. Specific Aim 2: Genetic analysis of genes isolated in the above screens. Specific Aim 3: To map and clone mutants isolated in the screens.