Genetics of postdauer developmental timing in C elegans

描述(由申请人提供)：细胞分裂和分化在不同组织之间以及有机体与环境之间的协调时间是正常生长的关键方面。这些过程的控制很重要，因为无论生长控制信号如何，细胞分裂都是癌症的标志。线虫中的异时性基因控制着发育过程中细胞分裂和分化的相对时间。这些基因的突变导致一些细胞谱系遗漏或重复部分发育程序，导致各种异常组织。然而，如果幼虫通过在不利条件下选择的另一种称为“Dauer”的途径发育，这些基因中的许多突变表型可能会被抑制。这表明，一些以前描述的异时基因对于后期发育是不必要的，这引发了哪些基因控制后期幼虫的发育时机的问题。该项目的目标是识别和表征这些基因。具体目的1：进行遗传筛选以分离发育计时后期缺陷突变体。具体目标2：对上述筛选中分离的基因进行遗传分析。具体目标3：对筛选到的突变体进行定位和克隆。

DESCRIPTION (provided by applicant): The coordinated timing of cell divisions and differentiation between different tissues and between the organism and the environment are critical aspects of normal growth. The control of these processes is important, as cell division irrespective of growth control signals is a hallmark of cancer. Heterochronic genes in C. elegans control the relative timing of cell divisions and differentiation during development. Mutations in these genes result in some cell lineages either omitting or reiterating parts of their developmental program, leading to a variety of abnormal tissues. However, the mutant phenotypes of many of these genes can be suppressed if larvae develop through an alternative pathway called "dauer", chosen in unfavorable conditions. This suggests that some previously characterized heterochronic genes are dispensable for postdauer development, raising the question of what genes control developmental timing in postdauer larvae. The objective of this project is to identify and characterize such genes. Specific Aim 1: To carry out genetic screens to isolate mutants defective in developmental timing postdauer. Specific Aim 2: Genetic analysis of genes isolated in the above screens. Specific Aim 3: To map and clone mutants isolated in the screens.