The acclimation of Chlamydomonas to sulfur deprivation.

衣藻对缺硫的适应。

• 批准号: 6938903
• 负责人: Steve V Pollock
• 金额: $ 0.83万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6938903
• 关键词: Chlamydomonas; biological signal transduction; genetic models; genetic strain; intermolecular interaction; model design /development; mutant; plant genetics; plant proteins; postdoctoral investigator; sulfur

DESCRIPTION (provided by applicant): This proposal describes a series of genetic and biochemical approaches that will be used to study the components of the sulfur-signal transduction cascade that is responsible for the acclimation responses of Chlamydomonas reinhardtii to sulfur deprivation. Initial work will generate a collection of insertional mutants that lack the normal responses to sulfur deprivation. Genetic analysis will be used to determine if the mutant phenotype segregates with the insertion. The genetic lesions in these mutants will be determined and complementation will be attempted by transforming the mutants with the wild-type locus. This information will be used to generate a model for the sulfate signal transduction cascade using a genetic approach. Using the pathway model, biochemical methods will investigate the possible interaction between components of an individual pathway, as well as to discover new interactors. The wild-type and mutant strains will be used for biochemical studies focused on determining which of the proteins involved in the acclimation of cells to sulfur deprivation physically interact. This will help build a more detailed picture of the signaling pathway.

描述（由申请人提供）：本提案描述了一系列遗传和生化方法，这些方法将用于研究负责莱茵衣藻对缺硫的适应反应的硫信号转导级联的组分。最初的工作将产生一系列缺乏对硫剥夺的正常反应的插入突变体。将使用遗传分析来确定突变表型是否与插入分离。将确定这些突变体中的遗传损伤，并通过用野生型基因座转化突变体来尝试互补。这些信息将被用来生成一个模型的硫酸盐信号转导级联使用遗传方法。使用通路模型，生物化学方法将研究单个通路的组分之间可能的相互作用，以及发现新的相互作用。野生型和突变株将用于生物化学研究，重点是确定哪些蛋白质参与细胞对硫剥夺的适应，并发生物理相互作用。这将有助于建立一个更详细的图片的信号通路。