Defining Emergence from Stationary Phase in Yeast

描述（由申请人提供）：本文提出的研究目的是了解静止细胞，以及芽殖酵母酿酒酵母中静止细胞和有丝分裂周期细胞之间的关系。在基因组学出现之前，不可能在转录组水平上研究这些问题。使用最近开发的快速采样技术，我们可以生成，第一次，精细分辨率的时间过程中的基因表达的研究细胞培养退出静止，并在指数分裂的细胞培养。为了检查静止细胞和与循环细胞的关系，我们将：1。研究稳定期培养物在细胞大小、DNA含量和基因表达方面的异质性，2）在同步分裂细胞中进行G1的快速取样，3.）从细胞退出稳定期开始进行更长的时间过程，以及4.）开发和使用多元曲线解析方法作为强大的基因组分析工具。通过了解与酵母静止有关的问题，我们可以对困扰人类的疾病获得重要的见解，例如由静止细菌病原体引起的慢性感染，以及在失去保持静止能力的细胞中观察到的致癌转化。

DESCRIPTION (provided by applicant): The objective of the research proposed here is to understand quiescent cells, and the relationship between quiescent cells and mitotically cycling cells in the budding yeast Saccharomyces cerevisiae. Prior to the advent of genomics, it was not possible to study these questions at the level of the transcriptome. Using recently developed rapid-sampling technology; we can generate, for the first time, fine-resolution time course studies of gene expression in cell cultures exiting quiescence, and in exponentially dividing cell cultures. To examine quiescent cells and the relationship to cycling cells we will: 1.) Investigate the heterogeneity of stationary phase cultures with respect to cell size, DNA content, and gene expression, 2.) Conduct a rapid sampling of G1 in synchronized dividing cells, 3.) Conduct a longer time course from cells exiting stationary phase, and 4.) Develop and use mutivariate curve resolution methods as a powerful genomic analysis tool. By understanding the questions related to quiescence in yeast, we can gain significant insights into diseases that afflict humans such as the chronic infections caused by quiescent bacterial pathogens, and the carcinogenic transformation observed in cells that have lost the capacity to remain quiescent.