Multi-keV x-ray microscopy facility for bio-imaging

描述（由申请人提供）：我们建议在斯坦福同步辐射实验室（SSRL）开发一个基于带板的多kev x射线成像设备，该设备将能够以20纳米分辨率成像厚水合生物标本，并使用层析成像重建提供三维图像。通过使用x射线相衬成像以及对比剂和特定标记，如免疫金、量子点或为电子显微镜开发的其他标记技术，该仪器将成为绘制细胞或组织细胞中特定分子的工具。随着带板光学的预期改进，我们期望分辨率最终达到或超过10边缘。该仪器旨在供生物医学研究界广泛使用，因此在其构建过程中，我们将展示其在两个截然不同的生物学问题上的功效：(1)绘制伤口组织中的微生物和大分子图；(2)确定水合骨的纳米结构。该提案结合了三个合作组织的专业知识：来自SSRL的P. Pianetta教授和K. Luening博士，他们在基于同步辐射源的各种x射线技术方面拥有丰富的经验；E. Almeida博士（加州大学旧金山分校，美国宇航局艾姆斯研究中心）和M. van der Meulen教授（康奈尔大学），他们是骨纳米结构研究的领导者；特伦特博士（美国宇航局艾姆斯研究中心），一位有成就的微生物学家，他在微生物成像和大分子方面做了大量工作，目前领导一个研究小组研究慢性伤口的微生物生态学。该显微镜将从Xradia公司购买，该公司是利用实验室x射线源产生的多kev x射线开发无损高分辨率x射线成像解决方案的领导者。Xradia创始人兼总裁云文兵博士将担任该项目的顾问，为真实生物标本开发高分辨率2D和3D相对比图像所需的技术。

DESCRIPTION (provided by applicant): We propose to develop a zone plate based, multi-keV x-ray imaging facility at the Stanford Synchrotron Radiation Laboratory (SSRL) that will be capable of imaging thick hydrated biological specimens with 20 nm resolution and provide three dimensional images using tomographic reconstruction. By using x-ray phase contrast imaging as well as contrasting agents and specific labels such as immunogold, quantum dots, or other labeling techniques developed for electron microscopy, the instrument will be a tool for mapping specific molecules in cells or cells in tissues. With the anticipated improvements in zone plate optics, we expect the resolution to ultimately reach or exceed 10 rim. This instrument is meant to be used by the biomedical research community at large, and therefore in its construction we will demonstrate its efficacy on two radically different biological problems: (1) mapping microbes and macromolecules in wound tissue and (2) determining the nanostructure of hydrated bone. This proposal combines the expertise of three collaborating organizations: Prof. P. Pianetta and Dr. K. Luening from SSRL with extensive experience in a wide range of x-ray techniques based on synchrotron radiation sources; Dr. E. Almeida (UCSF, NASA Ames Research Center) and Prof. M. van der Meulen (Cornell University) who are leaders in studies of the nanostructure of bone; and Dr. J. Trent (NASA Ames Research Center), an accomplished microbiologist, who has worked extensively in imaging microbes and macromolecules and currently heads a research team studying the microbial ecology of chronic wounds. The microscope will be purchased from Xradia Inc., a leader in developing nondestructive high-resolution x-ray imaging solutions using multi-keV x-rays produced by a laboratory x-ray source. Dr. Wenbing Yun, founder and president of Xradia, will serve as a consultant on the project to develop the techniques needed for achieving high resolution 2D and 3D images in phase contrast for real biological specimens.