Multi-keV x-ray microscopy facility for bio-imaging

用于生物成像的多 keV X 射线显微镜设备

• 批准号: 6847242
• 负责人: PIERO A PIANETTA
• 金额: $ 76.15万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6847242
• 关键词: X ray microscopy; bioimaging /biomedical imaging; biomedical facility; bone imaging /visualization /scanning; contrast media; infection; laboratory mouse; macromolecule; molecular /cellular imaging; scanning electron microscopy; technology /technique development; three dimensional imaging /topography; wound

DESCRIPTION (provided by applicant): We propose to develop a zone plate based, multi-keV x-ray imaging facility at the Stanford Synchrotron Radiation Laboratory (SSRL) that will be capable of imaging thick hydrated biological specimens with 20 nm resolution and provide three dimensional images using tomographic reconstruction. By using x-ray phase contrast imaging as well as contrasting agents and specific labels such as immunogold, quantum dots, or other labeling techniques developed for electron microscopy, the instrument will be a tool for mapping specific molecules in cells or cells in tissues. With the anticipated improvements in zone plate optics, we expect the resolution to ultimately reach or exceed 10 rim. This instrument is meant to be used by the biomedical research community at large, and therefore in its construction we will demonstrate its efficacy on two radically different biological problems: (1) mapping microbes and macromolecules in wound tissue and (2) determining the nanostructure of hydrated bone. This proposal combines the expertise of three collaborating organizations: Prof. P. Pianetta and Dr. K. Luening from SSRL with extensive experience in a wide range of x-ray techniques based on synchrotron radiation sources; Dr. E. Almeida (UCSF, NASA Ames Research Center) and Prof. M. van der Meulen (Cornell University) who are leaders in studies of the nanostructure of bone; and Dr. J. Trent (NASA Ames Research Center), an accomplished microbiologist, who has worked extensively in imaging microbes and macromolecules and currently heads a research team studying the microbial ecology of chronic wounds. The microscope will be purchased from Xradia Inc., a leader in developing nondestructive high-resolution x-ray imaging solutions using multi-keV x-rays produced by a laboratory x-ray source. Dr. Wenbing Yun, founder and president of Xradia, will serve as a consultant on the project to develop the techniques needed for achieving high resolution 2D and 3D images in phase contrast for real biological specimens.

描述（由申请人提供）：我们建议在斯坦福大学同步辐射实验室（SSRL）开发一种基于波带片的多keV X射线成像设备，该设备将能够以20 nm分辨率对厚水合生物样本进行成像，并使用断层扫描重建提供三维图像。通过使用X射线相衬成像以及造影剂和特定标记，如免疫金、量子点或其他为电子显微镜开发的标记技术，该仪器将成为绘制细胞或组织中细胞中特定分子的工具。随着波带片光学的预期改进，我们预计分辨率最终将达到或超过10 rim。该仪器旨在供生物医学研究界广泛使用，因此在其构建中，我们将证明其对两个完全不同的生物学问题的有效性：（1）绘制伤口组织中的微生物和大分子，以及（2）确定水合骨的纳米结构。该提案结合了三个合作组织的专业知识：P. Pianetta教授和K.来自SSRL的Luening博士在基于同步辐射源的各种X射线技术方面拥有丰富的经验;阿尔梅达（加州大学旧金山分校，美国宇航局艾姆斯研究中心）和M。货车der Meulen（康奈尔大学），他们是骨纳米结构研究的领导者;和J. Trent博士（NASA艾姆斯研究中心），一位有成就的微生物学家，他在微生物和大分子成像方面做了大量工作，目前领导一个研究慢性伤口微生物生态学的研究小组。显微镜将购自Xradia Inc.，在使用实验室X射线源产生的多keV X射线开发无损高分辨率X射线成像解决方案方面处于领先地位。Xradia的创始人兼总裁云文兵博士将担任该项目的顾问，开发为真实的生物标本实现高分辨率2D和3D相位对比图像所需的技术。