Biophysical Modeling of Solute Transport in Human IVD

人体 IVD 中溶质转运的生物物理模型

• 批准号: 6924392
• 负责人: Weiyong Gu
• 金额: $ 29.44万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6924392
• 关键词: backache; biological transport; biomechanics; biophysics; density; electrical conductance; electrochemistry; human tissue; intervertebral disk; mathematical model; mechanical stress; membrane permeability; nutrient bioavailability; oxygen tension; postmortem; spinal disk injury

DESCRIPTION (provided by applicant): Low back pain is a major socio-economic concern in this country. Although the exact cause for low back pain is unclear, the degenerative changes of the intervertebral disc (IVD) have been implicated as a possible primary etiologic factor. Poor nutritional supply is believed to be one of the mechanisms for disc degeneration. Due to the unique composition and structure of the materials and the complexity of the mechano-electrochemical coupling phenomena in IVD tissues, there is a lack of knowledge on transport properties of human IVDs or appropriate theoretical models for investigating nutrient transport in IVD systematically. The purpose of this application is to determine the transport properties and the constitutive models for these properties in human IVDs required for the development of a new mechano-electrochemical transport theory and finite element model in a subsequent grant application. The broad, long-term objectives of this project are to (1) elucidate the etiology of disc degeneration, (2) help develop strategies for restoring tissue function or retarding further disc degeneration, and (3) develop novel, less-invasive diagnostic tools for disc degeneration. In this research, we will determine the mechanical, physicochemical, and transport properties of human lumbar IVD tissues (Specific Aims #1-#5) and develop and validate new constitutive models for transport properties (Specific Aims #6 & #7). New technologies, based on mechano-electrochemical principles, will be developed for testing transport properties of IVD tissues, including strain-dependent hydraulic permeability, strain-dependent fixed charge density, strain-dependent electrical conductivity, and strain-dependent diffusivity of ions and nutrients (oxygen and glucose) in human lumbar discs. These properties will be correlated to the composition of the tissue, and will be used in developing the new constitutive theories in the research of nutritional supply in human IVDs. The advance in theory, knowledge on material properties, and techniques will have a significant impact on understanding the etiology of disc degeneration as well as on other areas of research, such as drug delivery in biological tissues. A plan for sharing and disseminating the theory, techniques, and data developed in this research is also included.

描述(由申请人提供)：腰痛是这个国家的一个主要的社会经济问题。虽然下腰痛的确切原因尚不清楚，但椎间盘退行性变(IVD)已被认为是可能的主要病因。营养供应不足被认为是腰椎间盘退变的机制之一。由于材料的独特组成和结构，以及IVD组织中机械力-电化学耦合现象的复杂性，目前还缺乏对人体IVD输运特性的了解，也没有合适的理论模型来系统研究IVD中的养分输运。本申请的目的是确定人体IVD中的传输特性和这些特性的本构模型，以便在随后的GRANT应用中开发新的机械-电化学传输理论和有限元模型。该项目的长期目标是(1)阐明椎间盘退变的病因，(2)帮助开发恢复组织功能或延缓进一步退变的策略，以及(3)开发新的、侵入性较小的诊断工具。在这项研究中，我们将确定人类腰椎IVD组织的力学、物理化学和传输特性(特定目标#1-#5)，并开发和验证新的传输特性本构模型(特定目标#6和#7)。基于机械-电化学原理，将开发新的技术来测试IVD组织的传输特性，包括应变相关的水力渗透性、应变相关的固定电荷密度、应变相关的电导率以及人体腰椎间盘中离子和营养物质(氧和葡萄糖)的应变相关扩散率。这些特性将与组织的组成相关联，并将用于开发新的本构理论，用于研究人体静脉畸形的营养供应。理论、材料特性和技术方面的进展将对理解椎间盘退变的病因以及其他研究领域(如生物组织中的药物释放)产生重大影响。还包括一项分享和传播本研究中开发的理论、技术和数据的计划。