Novel Quantitative Technology for Tissue Engineering

组织工程的新型定量技术

• 批准号: 7911620
• 负责人: Weiyong Gu
• 金额: $ 37万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7911620
• 关键词: Affect; Applications Grants; Area; Back; Biological; Bioreactors; Cell Density; Cell Proliferation; Cells; Cellular biology; Charge; Chemicals; Computer Simulation; Computing Methodologies; Connective Tissue; Consumption; Controlled Environment; Development; Electric Conductivity; Engineering; Environment; Etiology; Failure; Feedback; Flow Cytometry; Glucose; Goals; Growth; Histocompatibility Testing; In Vitro; Institution; Intervertebral disc structure; Ions; Knowledge; Laws; Liquid substance; Measurement; Measures; Mechanics; Metabolic; Methods; Modeling; Monitor; Nerve Tissue; Nutrient; Peripheral Nerves; Permeability; Physiological; Property; Research Activity; Signal Transduction; Stimulus; Strategic Planning; Stress; System; Techniques; Technology; Testing; Theoretical model; Tissue Engineering; Tissues; Water; Work; adult stem cell; base; density; design; design and construction; experience; feeding; foot; implantation; in vivo; innovation; new technology; novel; pressure; response; solute; technology development; theories; tool

Knowledge of the mechano-electrochemical signals around the cells and solute transport within a tissue is crucial to understanding cell biology, tissue growth and remodeling in vivo or in vitro. Bioreactors are essential for tissue engineering in vitro as the environment of engineered tissue construct could be controlled. Computer simulations are useful for estimation and prediction of the physical signals within the tissue or tissue construct if the environment of the tissue and the tissue properties are known. However, there is virtually no technology which is capable of online characterizing mechano-electrochemical properties of engineered tissue, or controlling physical signals within the tissue. The proposed research activities will fill the gap in this area. The longterm goals of this study are (1) to engineer functional tissues in vitro for implantation in vivo, and (2) to understand the relationship between mechano-chemical environment and tissue growth (Le., growth laws) for connective (e,g" intervertebral disc) tissues. The objective of this proposed project is to develop novel, bioreactor technology that could online monitor the changes in tissue properties as tissue growth in the bioreactor. In this project, a new bioreactor system will be designed and fabricated. New methods and techniques for characterizing of mechano-electrochemical properties of the engineered tissue will be developed.

了解细胞周围的机械电化学信号和组织内溶质运输对于理解体内或体外细胞生物学、组织生长和重塑至关重要。生物反应器对于体外组织工程至关重要，因为工程组织构建的环境是可以控制的。如果组织的环境和组织特性已知，则计算机模拟可用于估计和预测组织或组织构建体内的物理信号。然而，实际上没有任何技术能够在线表征工程组织的机械电化学特性，或控制组织内的物理信号。拟议的研究活动将填补该领域的空白。本研究的长期目标是（1）在体外设计功能组织以植入体内，以及（2）了解结缔组织（例如椎间盘）的机械化学环境与组织生长（即生长规律）之间的关系。该项目的目标是开发新颖的生物反应器技术，该技术可以在线监测生物反应器中组织生长时组织特性的变化。 项目中，将设计和制造一个新的生物反应器系统。将开发表征工程组织的机械电化学特性的新方法和技术。

项目成果

Mechanical loading affects the energy metabolism of intervertebral disc cells.

• DOI: 10.1002/jor.21430
• 发表时间: 2011-11
• 期刊: JOURNAL OF ORTHOPAEDIC RESEARCH
• 影响因子: 2.8
• 作者: Fernando, Hanan N.;Czamanski, Jessica;Yuan, Tai-Yi;Gu, Weiyong;Salahadin, Abdi;Huang, Chun-Yuh Charles
• 通讯作者: Huang, Chun-Yuh Charles

Cell viability in intervertebral disc under various nutritional and dynamic loading conditions: 3d finite element analysis.

• DOI: 10.1016/j.jbiomech.2012.08.044
• 发表时间: 2012-11-15
• 期刊: Journal of biomechanics
• 影响因子: 2.4
• 作者: Zhu Q;Jackson AR;Gu WY
• 通讯作者: Gu WY

A new constitutive model for hydration-dependent mechanical properties in biological soft tissues and hydrogels.

• DOI: 10.1016/j.jbiomech.2014.06.012
• 发表时间: 2014-09-22
• 期刊: JOURNAL OF BIOMECHANICS
• 影响因子: 2.4
• 作者: Gao, Xin;Gu, Weiyong
• 通讯作者: Gu, Weiyong

Simulation of the progression of intervertebral disc degeneration due to decreased nutritional supply.

• DOI: 10.1097/brs.0000000000000560
• 发表时间: 2014-11-15
• 期刊: Spine
• 影响因子: 3
• 作者: Gu W;Zhu Q;Gao X;Brown MD
• 通讯作者: Brown MD

Temporal changes of mechanical signals and extracellular composition in human intervertebral disc during degenerative progression.

• DOI: 10.1016/j.jbiomech.2014.09.004
• 发表时间: 2014-11-28
• 期刊: JOURNAL OF BIOMECHANICS
• 影响因子: 2.4
• 作者: Zhu, Qiaoqiao;Gao, Xin;Gu, Weiyong
• 通讯作者: Gu, Weiyong