Novel Chemoenzymatic Approaches to 'Unnatural' Products

许多抗癌、抗生素、抗寄生虫和抗病毒药物的天然产物是在微生物中由多功能酶生物合成的，例如聚酮合成酶(PKS)和非核糖体多肽合成酶(NRPS)。这些自然产生的‘分子工厂’被视为开发化学酶途径产生‘非天然’产品的有希望的起点--这些分子仍然是通过生物合成产生的，但在结构上与原始产品不同，可能具有同等或更高的价值。这个博士项目的目的是开发新的化学酶方法来体外产生非天然产品：通过利用化学工具和工程酶/途径，我们的目标是建立具有制药和工业兴趣的新化合物的库。“

"Many natural products of anticancer, antibiotic, antiparasitic and antiviral agents, amongst others, are biosynthesised in microorganisms by multifunctional enzymes, such as the polyketide synthases (PKSs) and the nonribosomal peptide synthetases (NRPSs). These naturally occurring 'molecular factories' are seen as the promising starting point for the development of chemoenzymatic routes towards the generation of 'unnatural' product- molecules that are still produced via biosynthesis but that are structurally different from the original products and may be of equal or superior value.The aim of this PhD project is to develop novel chemoenzymatic approaches towards the in vitro generation of unnatural products: by utilising chemical tools and engineered enzymes/pathways, we aim at generating libraries of novel compounds of pharmaceutical and industrial interest."