Electro-injection of IL-12 DNA to Muscle for Cancer
将 IL-12 DNA 电注射至肌肉治疗癌症
基本信息
- 批准号:6943029
- 负责人:
- 金额:$ 32.71万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-08-09 至 2007-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant): To realize the promise of gene therapy as a treatment
modality for cancer, we must develop simplified but effective gene delivery approaches and understand how therapeutic proteins act to kill tumors. Our proposal directly addresses both of these critical elements. We have pioneered the use of muscle-based electroporation as a novel and inexpensive approach for simplified and effective gene delivery and have demonstrated that injection of the IL-12 gene to muscle accompanied by electroporation substantially enhances the level of IL-12 expression and its systemic antitumor efficacy, compared with injection without electroporation, leading to 20% eradication of tumors located distant to the gene injection site. We hypothesize that delivery of IL-12 DNA via intramuscular electroporation, in synergy with a novel muscle-specific expression system, will eradicate or inhibit remote squamous cell carcinoma (SCC) by upregulation of antitumor genes. Our long-term goal is to develop a simple and effective strategy for eradicating inaccessible small-load metastatic SCC or residual microscopic SCC after surgery. We will use a tumor model of SCC, known as SCCVII, which allows the generation of both subcutaneous and metastatic tumors in a syngeneic murine host (C3H/HeJ) to explore the following Specific Aims. Aim 1: Determine the optimal delivery and expression system for electroporation to achieve therapeutic levels of IL-12 in vivo, and assess the systemic toxicity of gene therapy in comparison to protein therapy. Aim 2: Determine the role of Stat 1 and primary immune cells in inhibition of tumor growth elicited by IL-12. This work holds enormous potential for progress in eradicating SCC, particularly in those clinical
situations where small-load metastases are inaccessible or in cases where residual microscopic
disease persists after surgery. The experiments that we propose have a high probability of success because we have an excellent model system in place, experience in the electroporation gene therapy field, and all the resources necessary to complete the task.
描述(由申请人提供):实现基因治疗作为一种治疗方法的承诺
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('SHULIN LI', 18)}}的其他基金
Tumor-Targeted Chemo-Immunotherapy for Osteosarcoma Metastasis
骨肉瘤转移的肿瘤靶向化学免疫治疗
- 批准号:
9175963 - 财政年份:2016
- 资助金额:
$ 32.71万 - 项目类别:
Tumor-Targeted Chemo-Immunotherapy for Osteosarcoma Metastasis
骨肉瘤转移的肿瘤靶向化学免疫治疗
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9294022 - 财政年份:2016
- 资助金额:
$ 32.71万 - 项目类别:
Tumor-Targeted Chemo-Immunotherapy for Osteosarcoma Metastasis
骨肉瘤转移的肿瘤靶向化学免疫治疗
- 批准号:
9926814 - 财政年份:2016
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$ 32.71万 - 项目类别:
Low Electric Field-Based Gene Delivery of IL-30 Gene Therapy for Liver Injury
基于低电场的 IL-30 基因递送基因治疗肝损伤
- 批准号:
9254540 - 财政年份:2015
- 资助金额:
$ 32.71万 - 项目类别:
Low Electric Field-Based Gene Delivery of IL-30 Gene Therapy for Liver Injury
基于低电场的 IL-30 基因递送基因治疗肝损伤
- 批准号:
9050669 - 财政年份:2015
- 资助金额:
$ 32.71万 - 项目类别:
Low Electric Field-Based Gene Delivery of IL-30 Gene Therapy for Liver Injury
基于低电场的 IL-30 基因递送基因治疗肝损伤
- 批准号:
8882981 - 财政年份:2015
- 资助金额:
$ 32.71万 - 项目类别:
IL12 Gene Therapy For Enhancing Therapeutic Efficacy of Bleomycin Against Oral Tu
IL12基因疗法增强博来霉素抗口腔肿瘤的疗效
- 批准号:
8512669 - 财政年份:2010
- 资助金额:
$ 32.71万 - 项目类别:
IL12 Gene Therapy For Enhancing Therapeutic Efficacy of Bleomycin Against Oral Tu
IL12基因疗法增强博莱霉素抗口腔肿瘤的疗效
- 批准号:
8142751 - 财政年份:2010
- 资助金额:
$ 32.71万 - 项目类别:
IL12 Gene Therapy For Enhancing Therapeutic Efficacy of Bleomycin Against Oral Tu
IL12基因疗法增强博来霉素抗口腔肿瘤的疗效
- 批准号:
8699156 - 财政年份:2010
- 资助金额:
$ 32.71万 - 项目类别:
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