Cell Culture and Tissue Morphology Core

细胞培养亚核心将产生和维持来自不同组织和来源的原代培养和细胞系，包括人类癌症、基因敲除小鼠的肿瘤和来自转基因小鼠的小鼠胚胎成纤维细胞。我们的任务是建立和鉴定人和小鼠胃肠道肿瘤/癌细胞系，以探索胃肠道肿瘤发生的细胞和分子机制。胃肠道肿瘤研究的主要障碍之一是缺乏来源于胃肠道组织的正常上皮细胞系。为了解决这一缺陷，亚核将提供一种服务，从胃肠道不同部分的转基因小鼠的胃肠道不同部分培养上皮细胞系，这些转基因小鼠的基因缺失与胃肠道肿瘤/癌症的发展有关。我们还将从包括胰腺、胃和肝脏在内的人类胃肠道组织的不同部分培养上皮细胞系。最后，亚核负责大规模重组病毒库存的生成和维护。组织形态分核心为该计划提供全面的组织病理学和肿瘤获取服务。CORE的任务是：1)分别从乔治敦大学医院(GUH)进行的外科手术或从动物模型CORE(CORE A)产生的动物模型中获取人和胃肠道肿瘤/正常组织(肝细胞、胰腺和胃)-新鲜的、快速冷冻的和石蜡包埋的组织，并将这些肿瘤和正常组织分配给项目的所有四个项目以及细胞培养分核心以产生细胞系；2)维护肿瘤库，其中将包括乔治敦大学(GU)广泛的Gl肿瘤内部收集，以及个别项目负责人为加强现有GU收集而获得的额外组织(例如，Byers博士-项目2获得的新加坡冰冻和正常肝细胞癌标本(SingHealth HCC标本)，以及L.Mishra博士获得的多伦多患病儿童医院BWS肿瘤的广泛收集--项目1)；3)提供全面的组织学服务，包括最先进的组织微阵列和免疫组织化学；以及4)在使用人类和动物样本进行和解释实验方面提供专家协助。这两个次级核心将通过与每个项目负责人以及其他核心(动物模型核心-核心A和行政核心-核心C)密切合作，以确保获得/存档/冷冻适当的组织并产生必要的人类和小鼠细胞系，从而在该计划中发挥协同中心的作用。

The Cell Culture Subcore will generate and maintain primary cultures and cell lines from various tissues and origins including human cancers, tumors in knockout mice, and mouse embryonic fibroblasts derived from genetically modified mice. Our mission is to generate and characterize human and mouse gastrointestinal tumor/cancer cell lines, which will be utilized for exploring the cellular and molecular mechanisms in gastrointestinal carcinogenesis. One of the major impediments in gastrointestinal cancer research has been the lack of normal epithelial cell lines derived from gastrointestinal tissues. To address this deficiency, the subcore will provide a service in developing epithelial cell lines from the different segments of the gastrointestinal tract of transgenic mice with gene deletions known to be associated with gastrointestinal tumor/cancer development. We will also generate epithelial cell lines from the different segments of human gastrointestinal tissues including pancreas, stomach and liver. Finally, the subcore is responsible for generation and maintenance of large-scale stocks of recombinant virus. The Tissue Morphology Subcore provides to the Program a comprehensive histopathology and tumor acquisition service. The mission of the core is to: 1) acquire human and gastrointestinal tumor/normal tissues (hepatocellular, pancreatic, and gastric)-fresh, snap frozen and paraffin embedded-from surgical procedures performed at Georgetown University Hospital (GUH) or from animal models generated by the animal models core (core A), respectively, and distribute these tumor and normal tissues to all four projects of the program as well as to the Cell Culture Subcore to generate cell lines; 2) maintain the tumor bank that will include the Georgetown University (GU) extensive in house collections of Gl tumors, as well as additional tissues obtained by the individual project leaders to enhance the existing GU collections (e.g. The Singapore collection of frozen and normal HCC specimens with compete clinical follow up (SingHealth HCC specimens) obtained by Dr. Byers-Project 2 and the Toronto Hospital for Sick Children's extensive collection of BWS tumors obtained by Dr. L. Mishra-Project 1); 3) provide a comprehensive tissue histological services, including state of the art tissue microarray and immunohistochemistry; and 4) provide expert assistance in conducting and interpreting experiments using specimens from human and animal subjects. Both subcores will function as a synergistic hub in the Program by working closely with each project leader as well as with the other cores (Animal Models Core-Core A and Administrative Core-Core C) to insure that the proper tissues are obtained/archived/frozen and the necessary human and mouse cell lines are generated.