Cell Culture and Tissue Morphology Core

细胞培养和组织形态学核心

• 批准号: 8744879
• 负责人: SHULIN LI
• 金额: $ 15.22万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8744879
• 关键词: Address; Animal Model; Animals; Archives; Breeding; CDK4 gene; Cancer cell line; Cell Culture Techniques; Cell Line; Cells; Charge; Child; Clinical; Clinical Research; Collection; Complementary DNA; Cyclin-Dependent Kinase Inhibitor 2A; Databases; Development; Electronic Mail; Embryo; Epithelial; Epithelial Cells; Family; Fibroblasts; Freezing; Gastrointestinal Neoplasms; Gastrointestinal tract structure; Gene Deletion; Gene Mutation; Generations; Genes; Hepatic; Histopathology; Hospitals; Housing; Human; Human Cell Line; Immunohistochemistry; Individual; Knockout Mice; Laboratories; Liver; Maintenance; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Mission; Molecular; Morphology; Mouse Cell Line; Mucous Membrane; Mus; Mutate; Normal tissue morphology; Operative Surgical Procedures; Pancreas; Pancreatic carcinoma; Paraffin Embedding; Partner in relationship; Pattern; Pharmaceutical Preparations; Phenotype; Play; Primary carcinoma of the liver cells; Proteins; Reagent; Research Personnel; Role; Secure; Services; Signal Transduction; Simian virus 40; Singapore; Source; Specimen; Stomach; Stomach Carcinoma; Telomerase; Temperature; Tissue Microarray; Tissues; Transgenic Mice; Translating; Translational Research; Treatment Efficacy; Tumor Bank; Tumor Cell Line; Tumor Tissue; Universities; University Hospitals; Virus; Work; anticancer research; c-Myc Staining Method; carcinogenesis; expression vector; follow-up; gastrointestinal; interest; intestinal epithelium; meetings; member; mouse model; neoplastic cell; novel therapeutics; programs; recombinant virus; research study; temperature sensitive mutant; tissue/cell culture; tumor

The Cell Culture Subcore will generate and maintain primary cultures and cell lines from various tissues and origins including human cancers, tumors in knockout mice, and mouse embryonic fibroblasts derived from genetically modified mice. Our mission is to generate and characterize human and mouse gastrointestinal tumor/cancer cell lines, which will be utilized for exploring the cellular and molecular mechanisms in gastrointestinal carcinogenesis. One of the major impediments in gastrointestinal cancer research has been the lack of normal epithelial cell lines derived from gastrointestinal tissues. To address this deficiency, the subcore will provide a service in developing epithelial cell lines from the different segments of the gastrointestinal tract of transgenic mice with gene deletions known to be associated with gastrointestinal tumor/cancer development. We will also generate epithelial cell lines from the different segments of human gastrointestinal tissues including pancreas, stomach and liver. Finally, the subcore is responsible for generation and maintenance of large-scale stocks of recombinant virus. The Tissue Morphology Subcore provides to the Program a comprehensive histopathology and tumor acquisition service. The mission of the core is to: 1) acquire human and gastrointestinal tumor/normal tissues (hepatocellular, pancreatic, and gastric)-fresh, snap frozen and paraffin embedded-from surgical procedures performed at Georgetown University Hospital (GUH) or from animal models generated by the animal models core (core A), respectively, and distribute these tumor and normal tissues to all four projects of the program as well as to the Cell Culture Subcore to generate cell lines; 2) maintain the tumor bank that will include the Georgetown University (GU) extensive in house collections of Gl tumors, as well as additional tissues obtained by the individual project leaders to enhance the existing GU collections (e.g. The Singapore collection of frozen and normal HCC specimens with compete clinical follow up (SingHealth HCC specimens) obtained by Dr. Byers-Project 2 and the Toronto Hospital for Sick Children's extensive collection of BWS tumors obtained by Dr. L. Mishra-Project 1); 3) provide a comprehensive tissue histological services, including state of the art tissue microarray and immunohistochemistry; and 4) provide expert assistance in conducting and interpreting experiments using specimens from human and animal subjects. Both subcores will function as a synergistic hub in the Program by working closely with each project leader as well as with the other cores (Animal Models Core-Core A and Administrative Core-Core C) to insure that the proper tissues are obtained/archived/frozen and the necessary human and mouse cell lines are generated.

细胞培养亚核心将产生和维持来自各种组织和来源的原代培养和细胞系，包括人类癌症、基因敲除小鼠肿瘤和来自转基因小鼠的小鼠胚胎成纤维细胞。我们的任务是生成和表征人类和小鼠胃肠道肿瘤/癌细胞系，这将用于探索胃肠道癌变的细胞和分子机制。胃肠道肿瘤研究的主要障碍之一是缺乏来自胃肠道组织的正常上皮细胞系。为了解决这一缺陷，该亚核心将提供一种服务，用于开发来自胃肠道不同部分的上皮细胞系，这些基因缺失已知与胃肠道肿瘤/癌症的发展相关。我们还将从人体胃肠道组织的不同部分，包括胰腺、胃和肝脏中产生上皮细胞系。最后，亚核负责产生和维持重组病毒的大规模储存。组织形态学亚核心为该计划提供全面的组织病理学和肿瘤采集服务。核心的任务是：1)分别从乔治城大学医院（GUH）进行的外科手术中或从动物模型核心（核心A）生成的动物模型中获取新鲜、速冻和石蜡包埋的人类和胃肠道肿瘤/正常组织（肝细胞、胰腺和胃），并将这些肿瘤和正常组织分配给该计划的所有四个项目以及细胞培养亚核心以产生细胞系；2)维护肿瘤库，包括乔治城大学（GU）大量的Gl肿瘤收藏；以及由个别项目负责人获得的额外组织，以加强现有的GU收集（例如，byers博士项目2获得的新加坡冷冻和正常HCC标本（SingHealth HCC标本），以及L. mishra博士项目1获得的多伦多病儿医院广泛收集的BWS肿瘤）；3)提供全面的组织组织学服务，包括最先进的组织微阵列和免疫组织化学；4)在使用人类和动物受试者的标本进行和解释实验方面提供专家协助。这两个子核心都将作为项目的协同中心，与每个项目负责人以及其他核心（动物模型核心-核心a和管理核心-核心C）密切合作，以确保获得/存档/冷冻适当的组织，并生成必要的人类和小鼠细胞系。