Swinging Stomata: 4D capture of plant cell morphodynamics to inform on wall mechanic

表皮中相邻的保卫细胞（GC）收缩以关闭气孔，调节气体流入植物进行光合作用。通过BBSRC资助的工作，我们创建了一个有限元（FE）模型，使我们能够测试细胞壁在这一过程中的作用（Woolfenden et al.，Curr. Biol 2017）。我们的模型是基于传统的观点，GC运动发生在表皮的平面。使用共聚焦成像和标记的GC的3D重建，我们现在已经表明关闭运动是复杂的，涉及围绕枢轴旋转和形状变化，因此GC实际上向下摆动以关闭孔。

Adjoining guard cells (GCs) in the epidermis deflate to close the stomatal pore, regulating gas flow into plants for photosynthesis. Via BBSRC-funded work we created a finite-element (FE) model, allowing us to test ideas on the role of cell wall in this process (Woolfenden et al., Curr. Biol 2017). Our model was based on the traditional view that GC movement occurs in the plane of the epidermis. Using confocal imaging and 3D reconstruction of labelled GCs, we have now shown that the closing motion is complex, involving rotation around a pivot and shape change, so the GCsactually swing down to shut the pore.