Instrumentation for HDL and LDL subclass measurements

HDL 和 LDL 亚类测量仪器

• 批准号: 6932760
• 负责人: DONALD HOLVE
• 金额: $ 9.99万
• 依托单位: PROCESS METRIX, LLC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932760
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; clinical biomedical equipment; high density lipoproteins; low density lipoprotein; protein quantitation /detection; protein structure

DESCRIPTION (provided by applicant): A new methodology based on gas-phase electrical mobility will be developed for measuring lipoprotein particle size distributions and will be applicable to subclass analysis. This is a technology development project and will lead to a new instrument that will resolve lipoprotein classes and subclasses. The technique is capable of quantitating lipoprotein particles, in term of mass and number, in each subclass. Currently available techniques for subclass analysis are too slow (gel electrophoresis) and too expensive (NMR spectrometry) to address the growing commercial interest in subclass analysis. If the cost of subclass analysis could be reduced, it could become a highly prescribed test because subclasses of lipoprotein particles are significantly more predictive than cholesterol tests for future coronary heart disease. Particle electrical mobility measurements reveal subclasses comparable to gradient gel electrophoresis results, but much more rapidly. Electrical mobility measuring instrumentation is much cheaper than NMR spectrometers the current obstacle confronting the widespread implementation of particle electrical mobility for subclass testing is the lack of a commercial high throughput instrument. A redesigned instrument with improved size resolution will help to make the methodology acceptable. We demonstrated the methodology using differential electrical mobility analysis. We conceptualized a next generation gas-phase particle electrical mobility spectrometer for lipoprotein analysis and propose to evaluate its design for providing high throughput lipoprotein subclass analysis and quantification.

描述（由申请人提供）：将开发一种基于气相电迁移率的新方法，用于测量脂蛋白粒度分布，并适用于亚类分析。这是一个技术开发项目，将导致一个新的仪器，将解决脂蛋白类和子类。该技术能够定量每个亚类中脂蛋白颗粒的质量和数量。目前可用的亚类分析技术太慢（凝胶电泳）和太昂贵（NMR光谱法），以解决日益增长的商业兴趣的亚类分析。如果亚类分析的成本可以降低，它可能成为一个高度规定的测试，因为脂蛋白颗粒的亚类比胆固醇测试更能预测未来的冠心病。粒子电迁移率测量揭示了亚类梯度凝胶电泳的结果，但更迅速。电迁移率测量仪器比NMR光谱仪便宜得多，目前广泛实施用于子类测试的粒子电迁移率所面临的障碍是缺乏商业化的高通量仪器。一个重新设计的仪器，具有更高的尺寸分辨率，将有助于使该方法可以接受。我们证明了使用差分电迁移率分析的方法。我们概念化的下一代气相粒子电迁移谱仪脂蛋白分析，并建议评估其设计提供高通量脂蛋白亚类分析和定量。