Instrumentation for HDL and LDL subclass measurements

描述(由申请人提供)：将开发一种基于气相电迁移率的新方法来测量脂蛋白颗粒分布，并将适用于亚类分析。这是一个技术开发项目，将导致一种新的仪器，将分解脂蛋白类别和亚类。这项技术能够根据质量和数量对每个亚类中的脂蛋白颗粒进行定量。目前可用的亚类分析技术太慢(凝胶电泳法)和太昂贵(核磁共振光谱学)，不能满足亚类分析日益增长的商业兴趣。如果亚类分析的成本能够降低，它可能会成为一种高度规范的测试，因为亚类脂蛋白颗粒比胆固醇测试对未来冠心病的预测性要强得多。颗粒电迁移率测量显示亚类可与梯度凝胶电泳结果相媲美，但速度更快。电迁移率测量仪器比核磁共振谱仪便宜得多，目前广泛应用于亚类测试的粒子电迁移率面临的障碍是缺乏商业化的高通量仪器。重新设计的仪器具有更高的尺寸分辨率，将有助于使该方法可接受。我们使用差分电迁移率分析对该方法进行了演示。我们提出了用于脂蛋白分析的新一代气相粒子电迁移率光谱仪的概念，并建议对其设计进行评估，以提供高通量的脂蛋白亚类分析和定量。

DESCRIPTION (provided by applicant): A new methodology based on gas-phase electrical mobility will be developed for measuring lipoprotein particle size distributions and will be applicable to subclass analysis. This is a technology development project and will lead to a new instrument that will resolve lipoprotein classes and subclasses. The technique is capable of quantitating lipoprotein particles, in term of mass and number, in each subclass. Currently available techniques for subclass analysis are too slow (gel electrophoresis) and too expensive (NMR spectrometry) to address the growing commercial interest in subclass analysis. If the cost of subclass analysis could be reduced, it could become a highly prescribed test because subclasses of lipoprotein particles are significantly more predictive than cholesterol tests for future coronary heart disease. Particle electrical mobility measurements reveal subclasses comparable to gradient gel electrophoresis results, but much more rapidly. Electrical mobility measuring instrumentation is much cheaper than NMR spectrometers the current obstacle confronting the widespread implementation of particle electrical mobility for subclass testing is the lack of a commercial high throughput instrument. A redesigned instrument with improved size resolution will help to make the methodology acceptable. We demonstrated the methodology using differential electrical mobility analysis. We conceptualized a next generation gas-phase particle electrical mobility spectrometer for lipoprotein analysis and propose to evaluate its design for providing high throughput lipoprotein subclass analysis and quantification.