Goblet Cell Stimulation Via Electric Charge Flux & Field

通过电荷通量刺激杯状细胞

• 批准号: 6932880
• 负责人: BRADLEY D VEATCH
• 金额: $ 10万
• 依托单位: ADA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-15 至 2006-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932880
• 关键词: animal tissue; biomedical equipment development; cell proliferation; clinical biomedical equipment; conjunctiva; electric field; electrical potential; electrostimulus; goblet cells; keratoconjunctivitis sicca; mucins; therapy design /development; tissue /cell culture

DESCRIPTION (provided by applicant): Keratoconjunctivitus sicca (KCS), or dry eye, is a prevalent disease affecting an estimated one in five adults to varying degrees. Of Americans over the age of 65, some 4.3 million persons report tear film deficiencies, and certain diseases put patients at increased risk for the disease, including diabetes, lupus, arthritis, leukemia, fibromyalgia, acne rosacea, and Sjogren's syndrome. Significant research has focused on alleviating the effects and discomfort of dry eye, and new drugs and topical preparations are available. Results reported in the literature suggest that an external non-pharmacological treatment may be possible, based instead upon electrophysics. Dr. A. P. Krueger reports that tracheal tissues are stimulated to secrete mucus by exposure to negatively charged gaseous air ions. Dr. A. P. Wehner reports similar results for patients inhaling electro-aerosols. Finally, Dr. J. V. Forrester et al. have shown that electric fields play a significant role in ocular surface wound healing. Based upon these data, ADA believes that conjunctival goblet cells can be actively stimulated to secrete mucins and proliferate by subjecting them to an electric charge flux and electric field simultaneously. In Phase I, two experimental systems will be devised-one using coronal discharge and the other an electrospray technique-to supply calibrated levels of electric charge flux and electric field gradient to bovine conjunctival goblet cell cultures. Mucin secretion and proliferation will be evaluated to confirm or refute ADA's stimulation hypothesis. If confirmed, Phase II will incorporate in vivo testing to assess wound healing effects, anticipated stimulation of lacrimal and meibomian glands, and equipment development with the intent of commercializing a practical treatment system in Phase III.

描述（由申请人提供）：干燥性角结膜炎 (KCS) 或干眼症是一种常见疾病，估计五分之一的成年人在不同程度上受到影响。在 65 岁以上的美国人中，约有 430 万人报告泪膜缺陷，某些疾病使患者患病的风险增加，包括糖尿病、狼疮、关节炎、白血病、纤维肌痛、酒渣鼻和干燥综合征。重要的研究集中在减轻干眼症的影响和不适上，并且有新的药物和外用制剂可供使用。 文献报道的结果表明，基于电物理学的外部非药物治疗是可能的。 A. P. Krueger 博士报告说，气管组织通过暴露于带负电的气态空气离子而受到刺激而分泌粘液。 A. P. Wehner 博士报告了吸入电气雾剂的患者的类似结果。最后，J. V. Forrester 博士等人。已经表明电场在眼表伤口愈合中发挥着重要作用。根据这些数据，ADA 认为，通过同时使结膜杯状细胞受到电荷流和电场的作用，可以主动刺激结膜杯状细胞分泌粘蛋白并增殖。 在第一阶段，将设计两个实验系统，一个使用日冕放电，另一个使用电喷雾技术，为牛结膜杯状细胞培养物提供校准水平的电荷通量和电场梯度。将评估粘蛋白分泌和增殖以证实或反驳 ADA 的刺激假设。如果得到证实，第二阶段将包括体内测试，以评估伤口愈合效果、预期对泪腺和睑板腺的刺激，以及设备开发，以便在第三阶段将实用的治疗系统商业化。