Goblet Cell Stimulation Via Electric Charge Flux & Field

通过电荷通量刺激杯状细胞

• 批准号: 6932880
• 负责人: BRADLEY D VEATCH
• 金额: $ 10万
• 依托单位: ADA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-15 至 2006-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932880
• 关键词: animal tissue; biomedical equipment development; cell proliferation; clinical biomedical equipment; conjunctiva; electric field; electrical potential; electrostimulus; goblet cells; keratoconjunctivitis sicca; mucins; therapy design /development; tissue /cell culture

DESCRIPTION (provided by applicant): Keratoconjunctivitus sicca (KCS), or dry eye, is a prevalent disease affecting an estimated one in five adults to varying degrees. Of Americans over the age of 65, some 4.3 million persons report tear film deficiencies, and certain diseases put patients at increased risk for the disease, including diabetes, lupus, arthritis, leukemia, fibromyalgia, acne rosacea, and Sjogren's syndrome. Significant research has focused on alleviating the effects and discomfort of dry eye, and new drugs and topical preparations are available. Results reported in the literature suggest that an external non-pharmacological treatment may be possible, based instead upon electrophysics. Dr. A. P. Krueger reports that tracheal tissues are stimulated to secrete mucus by exposure to negatively charged gaseous air ions. Dr. A. P. Wehner reports similar results for patients inhaling electro-aerosols. Finally, Dr. J. V. Forrester et al. have shown that electric fields play a significant role in ocular surface wound healing. Based upon these data, ADA believes that conjunctival goblet cells can be actively stimulated to secrete mucins and proliferate by subjecting them to an electric charge flux and electric field simultaneously. In Phase I, two experimental systems will be devised-one using coronal discharge and the other an electrospray technique-to supply calibrated levels of electric charge flux and electric field gradient to bovine conjunctival goblet cell cultures. Mucin secretion and proliferation will be evaluated to confirm or refute ADA's stimulation hypothesis. If confirmed, Phase II will incorporate in vivo testing to assess wound healing effects, anticipated stimulation of lacrimal and meibomian glands, and equipment development with the intent of commercializing a practical treatment system in Phase III.

描述（由申请人提供）：干燥性角结膜炎（KCS）或干眼是一种流行病，估计五分之一的成年人会受到不同程度的影响。在65岁以上的美国人中，约有430万人报告泪膜缺陷，某些疾病使患者患这种疾病的风险增加，包括糖尿病、狼疮、关节炎、白血病、纤维肌痛、红斑痤疮和干燥综合征。重要的研究集中在减轻干眼症的影响和不适，并且可以获得新的药物和局部制剂。 文献中报告的结果表明，基于电物理学的外部非药物治疗是可能的。Dr. A. P.Krueger报道，通过暴露于带负电荷的气态空气离子，刺激气管组织分泌粘液。Dr. A. P. Wehner报告了吸入电气雾剂的患者的类似结果。最后，Dr. J. V. Forrester等人已经表明电场在眼表伤口愈合中起重要作用。基于这些数据，ADA认为，结膜杯状细胞可以通过使它们同时经受电荷通量和电场而被积极刺激以分泌粘蛋白并增殖。 在第一阶段，将设计两个实验系统，一个使用电晕放电和其他电喷雾技术，提供校准水平的电荷通量和电场梯度牛结膜杯状细胞培养。将评价粘蛋白分泌和增殖，以证实或反驳ADA的刺激假说。如果得到证实，第二阶段将包括体内测试，以评估伤口愈合效果，泪腺和睑板腺的预期刺激，以及旨在将第三阶段的实用治疗系统商业化的设备开发。