Photon-counting X-ray detector for Crystallography

用于晶体学的光子计数 X 射线探测器

• 批准号: 6883112
• 负责人: NEAL EUGENE HARTSOUGH
• 金额: $ 10万
• 依托单位: DXRAY, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6883112
• 关键词: X ray crystallography; bioimaging /biomedical imaging; electromagnetic radiation; high throughput technology; macromolecule; protein structure; synchrotrons; technology /technique development

DESCRIPTION (provided by applicant): The use of synchrotron x-ray diffraction techniques to determine the atomic structures of protein crystals has become a well-established technique over the last decade for both scientific and industrial biological and pharmaceutical applications. Thousands of complex molecular structures have been deduced over the past decade, leading to a tremendous increase in pharmaceutical drug development, and an increase in the understanding of enzyme and other biological processes. The x-ray diffraction techniques to elucidate the molecular structures are used in combination with chemical analysis techniques that derive the chemical and biological function of the molecules. New demands are now being placed on the synchrotron macromolecular crystallography facilities by researchers and companies who propose to investigate hundreds of protein structures in a short time period with high-throughput data collection. For the study of large unit cell crystals, detectors with larger areas than are currently available are required. In addition, to study time-dependent phenomena, faster detectors are also required. The current-integrating signal-processing techniques now being used will need to be replaced with single photon-counting techniques, in order to achieve the higher sensitivities, larger dynamic range and faster data collection times that are being sought. We propose to develop an entirely new approach for detectors for macromolecular crystallography, by creating an integrated detector consisting of an Hgl2 thin film deposited directly onto CMOS imaging arrays. The Hgl2 film will act as a direct conversion medium for the diffracted x-rays. The charge carriers generated in the Hgl2 from the absorbed x-rays are sensed at the CMOS sensor as induced charge, then amplified and processed.

描述（由申请人提供）：在过去十年中，使用同步加速器X射线衍射技术来确定蛋白质晶体的原子结构已成为科学和工业生物学和制药应用的成熟技术。在过去的十年中，已经推导出了数千种复杂的分子结构，导致药物开发的巨大增长，以及对酶和其他生物过程的理解的增加。用于阐明分子结构的X射线衍射技术与衍生分子的化学和生物学功能的化学分析技术结合使用。研究人员和公司现在对同步加速器大分子晶体学设施提出了新的要求，他们建议在短时间内通过高通量数据收集研究数百种蛋白质结构。对于大晶胞晶体的研究，需要比目前可用的面积更大的探测器。此外，为了研究与时间相关的现象，还需要更快的探测器。目前使用的电流积分信号处理技术将需要用单光子计数技术取代，以便实现正在寻求的更高灵敏度、更大动态范围和更快的数据收集时间。我们建议开发一种全新的方法，用于大分子晶体学检测器，通过创建一个集成的检测器组成的汞薄膜直接沉积到CMOS成像阵列。Hgl 2薄膜将作为衍射X射线的直接转换介质。Hgl 2中吸收的X射线产生的电荷载流子在CMOS传感器处被感测为感应电荷，然后被放大和处理。