Zebrafish Screen for Neuroprotective Anti-oxidants

斑马鱼筛选神经保护性抗氧化剂

• 批准号: 6880189
• 负责人: Chuenlei Parng
• 金额: $ 17.12万
• 依托单位: PHYLONIX PHARMACEUTICALS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6880189
• 关键词: acridines; antioxidants; apoptosis; drug screening /evaluation; fluorescence microscopy; free radicals; method development; microinjections; morphometry; neural degeneration; neuroprotectants; nonmammalian vertebrate embryology; oxidative stress; terminal nick end labeling; zebrafish

DESCRIPTION (provided by applicant): Neuronal systems are sensitive to oxidation. Oxidative damage to biochemical structures of nerve cells has been identified in a large number of neurodegenerative diseases, including stroke, brain trauma, spinal cord injury, Parkinson's disease, amyotrophic lateral sclerosis, Alzheimer's and Huntington's disease. These diseases are associated with high morbidity and mortality, and treatment options are limited. Development of agents that modulate oxidation is a major focus of biopharmaceutical research. Using zebrafish, this SBIR application aims to develop an in vivo system for screening potential neuroprotective anti-oxidants. Using visual assays, microscopy and image analysis, drug effects on neuronal apoptosis caused by oxidative damage will be assessed and quantified. Embryogenesis and patterning will also be assessed. Zebrafish is a good animal model for drug screening. During early development, zebrafish exhibits excellent optical clarity, making it possible to assess different cellular damage. Assessment of drug effects in a convenient vertebrate model, prior to evaluation in a complex system, such as mouse, can potentially streamline the drug development process and dramatically reduce costs.

描述（由申请人提供）： 神经元系统对氧化敏感。已经在许多神经退行性疾病中鉴定出对神经细胞生化结构的氧化损害，包括中风，脑外伤，脊髓损伤，帕金森氏病，氨基营养性的侧面硬化症，阿尔茨海默氏症和亨廷顿氏病。这些疾病与高发病率和死亡率有关，治疗方案受到限制。调节氧化的药物的开发是生物制药研究的主要重点。使用斑马鱼，该SBIR应用旨在开发一种体内系统，以筛选潜在的神经保护剂。使用视觉测定，显微镜和图像分析，将评估和量化药物对由氧化损伤引起的神经元细胞凋亡的影响。还将评估胚胎发生和模式。斑马鱼是药物筛查的好动物模型。在早期发育过程中，斑马鱼表现出极好的光学清晰度，使得评估不同的细胞损伤成为可能。在诸如小鼠等复杂系统中评估之前，在方便的脊椎动物模型中评估药物作用可以潜在地简化药物开发过程并大大降低成本。