Antimicrobial resistant bacteria and genes, drivers, and risk in the aquatic environments of Yorkshire

约克郡水生环境中的耐药细菌和基因、驱动因素和风险

• 批准号: 2596562
• 金额: --
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2596562
• 关键词: Antimicrobial; resistant; bacteria; genes; drivers

Antimicrobial resistance (AMR) is a global health threat, the aquatic environment is increasingly recognised as an important reservoir and source of transmission.The mixing of selection drivers, pathogenic and non-pathogenic bacteria increases the risk of antimicrobial resistance genes (ARGs) sharing between organism through Horizontal Gene Transfer (HGT) with the potential of producing resistant pathogens that could cause untreatable diseasesThe media frequently reports on the release of treated and untreated waste by water companies, but agriculture, healthcare, industrial and historic mining sites all contribute to driving the selection of AMR.There is currently no legal requirement to monitor AMR in discharge or the wider environment, research on rivers have been carried out in various countries including the UK but not in a Yorkshire River since 1971.The River Wharfe at Ilkley, Yorkshire was the first inland river site in the UK to be given formal bathing designation with an application for the River Nidd being assessed for approval in 2024, making both river catchments of particular interest.The project will consist of a 12-month assessment of antimicrobial resistant bacteria by culturing samples at multiple point on two rivers the Nidd and Wharfe to understand special-temporal fluctuations. During the bathing season, which currently only monitors faecal indicators, metagenomics analysis will be carried out to get an in-depth understanding of the river resistome. Chemical water quality characteristics including nitrogen, carbon, phosphorus and metals will also be collected. The data will then be evaluated to identify correlations between the measured variables, land use and predicted pollution sources.The conclusions can be used to inform local stakeholders including recreational water users, water companies and farmers, as well as contributing to future policy changes at higher levels.

抗生素耐药性（AMR）是一个全球性的健康威胁，水生环境越来越被认为是重要的储存库和传播源。选择驱动因素的混合，病原菌和非病原菌通过水平基因转移（HGT）增加了微生物之间共享抗菌素耐药基因（ARG）的风险具有产生可能导致无法治疗的疾病的抗性病原体的潜力媒体经常报道自来水公司释放处理过的和未处理的废物，但是农业，医疗保健，工业和历史采矿地点都有助于推动AMR的选择。目前没有法律的要求来监测排放或更广泛环境中的AMR，在包括英国在内的各个国家已经进行了对河流的研究，但自1971年以来没有在约克郡河中进行过研究。约克郡是英国第一个被正式指定为浴场的内陆河流，尼德河的申请将于2024年获得批准，该项目将包括一项为期12个月的评估，通过在Nidd和Wharfe两条河流的多个地点培养样本，对抗菌素耐药性细菌进行评估，以了解特殊的时间波动。在目前只监测粪便指标的沐浴季节，将进行宏基因组学分析，深入了解河流抗性基因组。此外，亦会收集水质的化学特性，包括氮、碳、磷和金属。然后，将对数据进行评估，以确定测量变量、土地使用和预测污染源之间的相关性，并将结论用于告知当地利益相关者，包括娱乐用水用户、水务公司和农民，以及为更高级别的未来政策变化做出贡献。