A Tissue-engineered, Small-diameter Vascular Graft

组织工程小直径血管移植物

• 批准号: 6904247
• 负责人: PAUL J DIMUZIO
• 金额: $ 12.86万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-19 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6904247
• 关键词: adipose tissue; bioengineering /biomedical engineering; biological models; biomaterial development /preparation; biomaterial interface interaction; biotechnology; blood vessel prosthesis; carotid artery; cell adhesion; clinical research; collagen; confocal scanning microscopy; dogs; extracellular matrix proteins; growth factor; hemodynamics; human subject; immunocytochemistry; integrins; medical implant science; tissue engineering; tissue support frame; trypsin; vascular endothelium

DESCRIPTION (provided by applicant): This proposal describes a five-year training program for the development of an academic career in vascular tissue engineering. The principal investigator is an accomplished vascular surgeon who has begun a basic science effort centered around the creation of a small-caliber vascular graft. Now, through a unique integration of interdepartmental resources, the principal investigator will expand his scientific skills to yield translational research. The program focuses on the creation and in vivo testing of a novel vascular graft. Irving Shapiro, PhD, Director of Jefferson's Cell and Tissue Engineering Program, will sponsor the principal investigator's scientific development along with Thomas Tulenko, PhD, Director of Jefferson's Cardiovascular Biology Research Program. Dr. Shapiro is a leader in the field of bone and cartilage tissue engineering, while Dr. Tulenko's expertise resides in vascular cell biology and cardiovascular disease. Both have extensive experience in training postdoctoral fellows and graduate students. To enhance the program, the investigator will work with Christopher Adams, PhD, an expert in electron and laser confocal microscopic analysis of tissue-engineered constructs; James San Antonio, PhD, an authority in endothelial cell-matrix interactions; and Yi Shi, MD, PhD, an expert in vascular graft remodeling. Keith Danielson, PhD, and Ronald Weigel, MD, PhD, scientists in stem cell engineering and cancer biology, respectively, will further advise and direct the investigator's educational program. The scientific goal of the project is to construct and test a novel small-caliber vascular bypass graft, created by seeding a natural vascular tissue scaffold (decellularized cadaver vein) with autologous microvessel endothelial cells derived from adipose tissue. The specific aims include determining: 1) the optimal scaffold design and media conditions for endothelial cell seeding, 2) the optimal paradigm for graft creation, and 3) the durability and healing characteristics of the seeded graft in vivo. Resources from the Jefferson's Cell and Tissue Engineering and Cardiovascular Research Laboratories provide the ideal setting for training physician-scientists in the field of vascular tissue engineering. This environment maximizes the potential for the principal investigator to achieve independence in a field with translational applicability.

描述（由申请人提供）： 该提案描述了一个为期五年的培训计划，在血管组织工程的学术生涯的发展。 主要研究者是一位有成就的血管外科医生，他已经开始了以创建小口径血管移植物为中心的基础科学工作。 现在，通过跨部门资源的独特整合，首席研究员将扩大他的科学技能，以产生转化研究。 该计划的重点是一种新型血管移植物的创建和体内测试。 欧文夏皮罗博士，杰斐逊的细胞和组织工程项目主任，将赞助主要研究者的科学发展沿着与托马斯Tulenko博士，杰斐逊的心血管生物学研究项目主任。 Shapiro博士是骨和软骨组织工程领域的领导者，而Tulenko博士的专长在于血管细胞生物学和心血管疾病。 两人在培养博士后研究员和研究生方面都有丰富的经验。 为了加强该计划，研究人员将与克里斯托弗亚当斯，博士，在电子和激光共聚焦显微镜分析的组织工程结构的专家;詹姆斯圣安东尼奥，博士，在内皮细胞-基质相互作用的权威;和易石，医学博士，博士，血管移植物重塑的专家。 干细胞工程和癌症生物学科学家基思丹尼尔森博士和罗纳德魏格尔医学博士将分别为研究者的教育计划提供进一步的建议和指导。 该项目的科学目标是构建和测试一种新型的小口径血管旁路移植物，通过将来自脂肪组织的自体微血管内皮细胞接种到天然血管组织支架（脱细胞尸体静脉）中来创建。 具体目标包括确定： 1)用于内皮细胞接种的最佳支架设计和培养基条件，2）用于移植物产生的最佳范例，和3）体内接种的移植物的耐久性和愈合特性。 来自杰斐逊细胞和组织工程以及心血管研究实验室的资源为培训血管组织工程领域的医生科学家提供了理想的环境。 这种环境最大限度地提高了主要研究者在具有转化适用性的领域实现独立性的潜力。