A Tissue-engineered, Small-diameter Vascular Graft

组织工程小直径血管移植物

• 批准号: 7124177
• 负责人: PAUL J DIMUZIO
• 金额: $ 12.86万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-19 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7124177
• 关键词: adipose tissue; bioengineering /biomedical engineering; biological models; biomaterial development /preparation; biomaterial interface interaction; biotechnology; blood vessel prosthesis; carotid artery; cell adhesion; clinical research; collagen; confocal scanning microscopy; dogs; extracellular matrix proteins; growth factor; hemodynamics; human subject; immunocytochemistry; integrins; medical implant science; tissue engineering; tissue support frame; trypsin; vascular endothelium

DESCRIPTION (provided by applicant): This proposal describes a five-year training program for the development of an academic career in vascular tissue engineering. The principal investigator is an accomplished vascular surgeon who has begun a basic science effort centered around the creation of a small-caliber vascular graft. Now, through a unique integration of interdepartmental resources, the principal investigator will expand his scientific skills to yield translational research. The program focuses on the creation and in vivo testing of a novel vascular graft. Irving Shapiro, PhD, Director of Jefferson's Cell and Tissue Engineering Program, will sponsor the principal investigator's scientific development along with Thomas Tulenko, PhD, Director of Jefferson's Cardiovascular Biology Research Program. Dr. Shapiro is a leader in the field of bone and cartilage tissue engineering, while Dr. Tulenko's expertise resides in vascular cell biology and cardiovascular disease. Both have extensive experience in training postdoctoral fellows and graduate students. To enhance the program, the investigator will work with Christopher Adams, PhD, an expert in electron and laser confocal microscopic analysis of tissue-engineered constructs; James San Antonio, PhD, an authority in endothelial cell-matrix interactions; and Yi Shi, MD, PhD, an expert in vascular graft remodeling. Keith Danielson, PhD, and Ronald Weigel, MD, PhD, scientists in stem cell engineering and cancer biology, respectively, will further advise and direct the investigator's educational program. The scientific goal of the project is to construct and test a novel small-caliber vascular bypass graft, created by seeding a natural vascular tissue scaffold (decellularized cadaver vein) with autologous microvessel endothelial cells derived from adipose tissue. The specific aims include determining: 1) the optimal scaffold design and media conditions for endothelial cell seeding, 2) the optimal paradigm for graft creation, and 3) the durability and healing characteristics of the seeded graft in vivo. Resources from the Jefferson's Cell and Tissue Engineering and Cardiovascular Research Laboratories provide the ideal setting for training physician-scientists in the field of vascular tissue engineering. This environment maximizes the potential for the principal investigator to achieve independence in a field with translational applicability.

描述（由申请人提供）： 该提案描述了一个为期五年的血管组织工程学术职业发展培训计划。 首席研究员是一位经验丰富的血管外科医生，他已经开始围绕小口径血管移植物的创建进行基础科学工作。 现在，通过跨部门资源的独特整合，首席研究员将扩展他的科学技能以产生转化研究。 该项目的重点是新型血管移植物的创建和体内测试。 杰斐逊细胞和组织工程项目主任 Irving Shapiro 博士将与杰斐逊心血管生物学研究项目主任 Thomas Tulenko 博士一起赞助首席研究员的科学发展。 Shapiro 博士是骨和软骨组织工程领域的领导者，而 Tulenko 博士的专业知识在于血管细胞生物学和心血管疾病。 两人在培养博士后和研究生方面都有丰富的经验。 为了加强该计划，研究人员将与克里斯托弗·亚当斯博士合作，克里斯托弗·亚当斯博士是组织工程结构的电子和激光共焦显微分析专家。 James San Antonio 博士，内皮细胞-基质相互作用领域的权威；石毅，MD，PhD，血管移植重塑专家。 分别是干细胞工程和癌症生物学科学家 Keith Danielson 博士和 Ronald Weigel 博士将进一步为研究者的教育计划提供建议和指导。 该项目的科学目标是构建和测试一种新型小口径血管旁路移植物，该移植物是通过在天然血管组织支架（脱细胞尸体静脉）中植入源自脂肪组织的自体微血管内皮细胞而创建的。 具体目标包括确定： 1) 内皮细胞接种的最佳支架设计和培养基条件，2) 移植物创建的最佳范例，以及 3) 体内接种移植物的耐久性和愈合特性。 杰斐逊细胞和组织工程以及心血管研究实验室的资源为培训血管组织工程领域的医师科学家提供了理想的环境。 这种环境最大限度地发挥了首席研究员在具有转化适用性的领域实现独立的潜力。