A Course in Computational Genomics for Experimentalists

项目描述（申请人提供）：本项目旨在开设一门生物信息学的讲座/实验课程，教授各种生物医学实验学科的研究生如何在他们的研究中分析和整合基因组数据。与其简单地提供一门生物信息学方法的课程，不如提出一套需要基因组分析来解决的说明性生物学问题。将开设一系列课程，涵盖将蛋白质和核酸序列与其结构、功能、进化、表达及其在人类健康和疾病中的作用联系起来的方法，教授所有研究方法的理论基础。使用的所有程序的操作将在液晶投影仪的帮助下进行现场演示。学生将在有监督的计算机实验室中，通过将所有方法应用于实际的生物学问题，获得自己使用所有方法的经验。大部分的实验练习将包括一个学期专题，学生将在其中探索参与细胞信号传导的蛋白质/基因家族的结构和功能。其他练习将包括通过连锁和序列分析的结合来寻找人类疾病基因，以及分析微阵列表达数据。重点将放在使用可通过网络访问的数据库。

DESCRIPTION (provided by applicant): The aim of this project is to develop a lecture/laboratory course in bioinformatics that teaches graduate students in the various experimental biomedical disciplines how to analyze and integrate genomic data in their research. Rather than simply offering a course in bioinformatic methods, it is proposed to develop a set of several illustrative biological problems that require genomic analysis for their solution, A series of lessons will be developed that cover the methods for relating the sequences of proteins and nucleic acids to their structure, function, evolution, expression, and role in human health and disease, The theoretical basis of all methods studied will be taught. The operation of all programs used will be demonstrated live, with the aid of an LCD projector. The students will gain experience in using all the methods themselves by applying them to real biological problems in a supervised computer laboratory. Most of the lab exercises will involve a term project in which the students explore the structure and function of a protein/gene family involved in cellular signaling. Other exercises will include finding a human disease gene by a combination of linkage and sequence analysis, and analyzing microarray expression data. Emphasis will be placed on the use of web-accessible databases.