Core-Biomodel

核心生物模型

• 批准号: 6781661
• 负责人: BARBARA M SCHREIBER
• 金额: $ 24.34万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6781661
• 关键词: animal colony; biomedical facility; cardiovascular injury; clinical research; confocal scanning microscopy; disease /disorder model; immunocytochemistry; in situ hybridization; laboratory mouse; laboratory rat; tissue /cell culture; tissue /cell preparation

The scientific Core with Dr. Schreiber as director will be responsible for services that are common to multiple projects within the program project. She has been serving as director of this Core during the current funding cycle. All Core services will be requested by requisition through Dr. Schreiber, who will approve such requests. Centralization of these services will lead to both procedural efficiency as well as cost efficiency. This Core will be responsible for 1) isolation, culturing and distribution of cell cultures and reagent preparation for cell cultures 2) implementation of the in vivo mouse femoral artery injury model common to all projects, 3) fixing, embedding and sectioning of cell and tissue samples 4.) in situ hybridizations and immunohistochemistry and 5) assisting in crossbreeding of mice. In addition, 6) confocal laser scanning microscopy (CLSM) will be performed by the individual investigators, the costs for which will be charged to the Core, which will also offer CLSM consulting services.

以Schreiber博士为主任的科学核心将负责以下服务： 是程序项目中的多个项目所共有的。她一直担任主任 在当前的供资周期内，所有核心服务将通过请购单提出请求 通过施赖伯博士，他会批准这些申请这些服务的集中化将 导致程序效率以及成本效率。该核心将负责1） 细胞培养物的分离、培养和分配以及细胞培养物的试剂制备2） 所有项目共同的体内小鼠股动脉损伤模型的实施，3） 细胞和组织样品的固定、包埋和切片4.）原位杂交， 免疫组化和5）辅助小鼠杂交。此外，6）共焦 激光扫描显微镜（CLSM）将由个体研究者进行， 这些费用将由核心支付，核心还将提供CLSM咨询服务。