Core-Biomodel

由施赖伯博士担任主任的科学核心将负责以下服务 是计划项目内的多个项目所共有的。她一直担任董事。 在当前的供资周期内，这一核心项目的执行情况将得到改善。所有核心服务都将按请购单请求 通过Schreiber博士，他将批准这类请求。这些服务的集中化将 既能提高程序效率，又能节约成本。此核心将负责1) 细胞培养物的分离、培养、分配及细胞培养物试剂制备2) 所有项目通用的在体小鼠股动脉损伤模型的实施，3) 细胞和组织标本的固定、包埋和切片。原位杂交和 免疫组织化学；5)协助小鼠杂交。此外，共焦 激光扫描显微镜(CLSM)将由个别调查人员进行， 费用将由Core支付，Core还将提供CLSM咨询服务。

The scientific Core with Dr. Schreiber as director will be responsible for services that are common to multiple projects within the program project. She has been serving as director of this Core during the current funding cycle. All Core services will be requested by requisition through Dr. Schreiber, who will approve such requests. Centralization of these services will lead to both procedural efficiency as well as cost efficiency. This Core will be responsible for 1) isolation, culturing and distribution of cell cultures and reagent preparation for cell cultures 2) implementation of the in vivo mouse femoral artery injury model common to all projects, 3) fixing, embedding and sectioning of cell and tissue samples 4.) in situ hybridizations and immunohistochemistry and 5) assisting in crossbreeding of mice. In addition, 6) confocal laser scanning microscopy (CLSM) will be performed by the individual investigators, the costs for which will be charged to the Core, which will also offer CLSM consulting services.