Core--GENOMICS

Genomics Core通过高通量自动化生产DNA微阵列，包括机器人生产和将cDNA或寡核苷酸点样到载玻片上。核心功能主要是为巴克研究人员和外部用户服务，包括微阵列实验的设计和分析。现有资源包括：用于小鼠和苍蝇的cDNA芯片，用于人类、小鼠和蠕虫的寡核苷酸芯片，以及用于制备、定位、杂交、定量和分析这些不同物种的微阵列芯片的适当仪器。通过将定量数据文件上传到Genetraffic（一种商业上可获得的软件包），完成对核心生成的数据的分析，这有助于通过密码保护系统远程检索相关数据，以及对差异基因表达进行初步分析。在过去的几年里，核心还发展了使用 学术上开发的统计分析模块，专门为微阵列数据的分析而设计。所有这些软件包都是免费的，并在计算环境“R”中开发和维护，并在生物导体网站（www.bioconductor.org）上列出。核心目前正在通过向一些实验室提供服务来促进研究所内的老龄化研究，这已经导致了一些出版物。巴克研究所基因组学核心目前还通过为与衰老和年龄相关疾病相关的研究提供微阵列专业知识和服务来为外部用户服务。

The Genomics Core produces DNA microarrays through high throughput automation, and involves robotic production and spotting of either cDNA or oligonucleotides onto glass slides. The core functions primarily to serve Buck investigators and outside users, in both the design and analysis of microarray experiments. The resources available include: cDNA chips for mice and fly, oligo chips for humans, mice, and worms, and the appropriate instrumentation to prepare, spot, hybridize, quantitate and analyze microarray chips from these diverse species. Archiving of data generated by the core is accomplished by uploading the quantitated data files into Genetraffic (a commercially available package), which facilitates remote retrieval of the relevant data via a password protected system, as well as rudimentary analysis of differential gene expression. Over the last several years, the core has also developed expertise in using academically developed statistical analysis modules, specifically designed for the analysis of microarray data. All these packages are free, and are developed and maintained in the computing environment "R", and listed at the bioconductor website (www.bioconductor.org).The Core is currently promoting aging research within the institute by providing service to a number of laboratories, and this has resulted in a several publications. The Buck Institute Genomics Core also currently serves outside users by providing microarray expertise and service for studies related to aging and age-related disease.