CORE--Leukocyte Migration

细胞黏附机制已被认为是调节靶向的关键因素 单个细胞到它们的功能部位。黏附决定因素决定了正常和异常皮肤成分在生长和伤口愈合过程中的迁移，肿瘤细胞的转移特性，以及感染反应和炎症性疾病发展过程中白细胞的聚集等关键特征。因此，细胞黏附特性的研究是皮肤病理生理学研究的核心。开发白细胞黏附核心的目的是为HSDRC的研究人员提供高质量的人类和小鼠白细胞黏附和迁移检测，并为这些检测的设计和执行提供专家帮助。过去五年对白细胞迁移核心资源的浓厚兴趣强化了我们的信念，即许多老牌和新的皮肤病研究科学家的研究得到了一个技术和智力中心的极大帮助，该中心可以帮助规划和执行细胞迁移和黏附实验。产生高质量的人类和小鼠白细胞黏附和迁移分析所需的相当大的费用以及必要的设置和培训时间是许多研究人员进入的障碍。核心主任的专业体外和体内技术以及丰富的经验将继续作为该核心对国家发改委调查人员价值的基础。通过实施新技术，包括新颖的体外流动分析和多光子共聚焦活体显微镜，以及更多地获得更传统的黏附和迁移分析技术，核心将继续为用户提供应用于他们的研究问题的尖端技术。此外，von Andrian博士设计的在T细胞和/或特定T细胞亚群中表达荧光蛋白的小鼠已经被培育出来并进行了鉴定，现在可供HSDRC的研究人员使用。白细胞迁移核心提供的服务可分为支持体外结合研究、体内归巢模型系统以及体内荧光和多光子显微镜技术。尽管存在相当大的重叠和合作，Fuhlbrigge博士将继续为哈佛医学院的体外核心服务提供主要支持，冯·安德里安博士将为血液研究中心的活体显微镜核心服务提供主要支持。整个动物体内归巢研究将继续在两个设施得到支持。

Cellular adhesion mechanisms have been recognized as crucial elements regulating the targeting of individual cells to their sites of function. Adhesion determinants direct such critical features as the migration of normal and abnormal skin components in growth and wound healing, the metastatic properties of tumor cells and as the accumulation of leukocytes in the response to infection and the development of inflammatory diseases. As such, investigation of cell adhesion properties is central to the study of skin pathophysiology. The Leukocyte Adhesion Core was developed to provide HSDRC investigators with ready access to high quality assays of human and murine leukocyte adhesion and migration and expert assistance with the design and execution of these assays. The strong interest in Leukocyte Migration Core resources over the past five years has reinforced our belief that the research of many established and new skin disease research scientists is aided significantly by a technical and intellectual center that can assist in the planning and execution of cell migration and adhesion experiments. The considerable expense and the setup and training time necessary to generate high quality assays of human and murine leukocyte adhesion and migration represents a barrier to entry for many investigators.The specialized in vitro and in vivo techniques and extensive experience of the Core Directors will continue to serve as the basis for the value this of this Core to SDRC investigators. Through the implementation of new technologies, including novel in vitro flow assays and multi-photon confocal intravital microscopy, along with expanded access to more conventional adhesion and migration assay techniques, the Core will continue to provide users with access to cutting edge technology for application to their research questions. In addition, mice engineered by Dr. von Andrian to express fluorescent proteins in T cells and/or specific T cell subpopulations, have been produced and characterized and are now available to HSDRC investigators. Services provided by the Leukocyte Migration Core are divisible into support for in vitro binding studies, in vivo homing model systems and intravital epifluorescence and multi-photon microscopy techniques. Although considerable overlap and collaboration exists, Dr. Fuhlbrigge will continue to provide primary support for in vitro core services at the Harvard Institutes of Medicine and Dr. von Andrian will provide primary support for intravital microscopy core services at the Center for Blood Research. Whole animal in vivo homing studies will continue to be supported in both facilities.