Leukocyte Migration

白细胞迁移

• 批准号: 7393284
• 负责人: ROBERT C FUHLBRIGGE
• 金额: $ 18.27万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7393284
• 关键词: Address; Adhesions; Animals; Area; Belief; Binding; Biological Assay; Biological Models; Blood; Blood capillaries; Bone Marrow; Cell Adhesion; Cells; Collaborations; Communities; Cutaneous; Development; Disease; Ear; Elements; Endothelium; Engineering; Equipment; Functional disorder; Growth; Homing; Human; In Vitro; Individual; Infection; Inflammation; Inflammatory; Institute of Medicine (U.S.); Instruction; Investigation; Laboratories; Lamina Propria; Leukocytes; Mesentery; Methods; Microscopy; Migration Assay; Modeling; Mus; Muscle; Nature; Peripheral; Play; Process; Property; Proteins; Protocols documentation; Regulation; Research; Research Personnel; Resources; Role; Scientist; Services; Site; Skin; Standards of Weights and Measures; Structure of aggregated lymphoid follicle of small intestine; T-Lymphocyte; Techniques; Technology; Time; Training; Wound Healing; base; capillary; cell motility; comparative; design; experience; in vivo; interest; intravital microscopy; leukocyte homing; lymph nodes; migration; multi-photon; neoplastic cell; new technology; novel; research study; response; shear stress; skin disorder

Cellular adhesion mechanisms have been recognized as crucial elements regulating the targeting of individual cells to their sites of function. Adhesion determinants direct such critical features as the migration of normal and abnormal skin components in growth and wound healing, the metastatic properties of tumor cells and as the accumulation of leukocytes in the response to infection and the development of inflammatory diseases. As such, investigation of cell adhesion properties is central to the study of skin pathophysiology. The Leukocyte Adhesion Core was developed to provide HSDRC investigators with ready access to high quality assays of human and murine leukocyte adhesion and migration and expert assistance with the design and execution of these assays. The strong interest in Leukocyte Migration Core resources over the past five years has reinforced our belief that the research of many established and new skin disease research scientists is aided significantly by a technical and intellectual center that can assist in the planning and execution of cell migration and adhesion experiments. The considerable expense and the setup and training time necessary to generate high quality assays of human and murine leukocyte adhesion and migration represents a barrier to entry for many investigators.The specialized in vitro and in vivo techniques and extensive experience of the Core Directors will continue to serve as the basis for the value this of this Core to SDRC investigators. Through the implementation of new technologies, including novel in vitro flow assays and multi-photon confocal intravital microscopy, along with expanded access to more conventional adhesion and migration assay techniques, the Core will continue to provide users with access to cutting edge technology for application to their research questions. In addition, mice engineered by Dr. von Andrian to express fluorescent proteins in T cells and/or specific T cell subpopulations, have been produced and characterized and are now available to HSDRC investigators. Services provided by the Leukocyte Migration Core are divisible into support for in vitro binding studies, in vivo homing model systems and intravital epifluorescence and multi-photon microscopy techniques. Although considerable overlap and collaboration exists, Dr. Fuhlbrigge will continue to provide primary support for in vitro core services at the Harvard Institutes of Medicine and Dr. von Andrian will provide primary support for intravital microscopy core services at the Center for Blood Research. Whole animal in vivo homing studies will continue to be supported in both facilities.

细胞粘附机制已被认为是调节靶向的关键因素 单个细胞到达其功能位点。粘附决定因素指导诸如生长和伤口愈合中正常和异常皮肤成分的迁移、肿瘤细胞的转移特性以及感染反应中白细胞的积累和炎症性疾病的发展等关键特征。因此，细胞粘附特性的研究是皮肤病理生理学研究的核心。白细胞粘附核心的开发是为了让 HSDRC 研究人员能够随时进行人类和小鼠白细胞粘附和迁移的高质量检测，并为这些检测的设计和执行提供专家协助。过去五年对白细胞迁移核心资源的浓厚兴趣增强了我们的信念，即许多成熟和新的皮肤病研究科学家的研究得到了技术和知识中心的大力帮助，该中心可以协助规划和执行细胞迁移和粘附实验。进行人类和小鼠白细胞粘附和迁移的高质量检测所需的大量费用以及设置和培训时间对许多研究人员来说构成了进入障碍。核心主任的专业体外和体内技术以及丰富的经验将继续作为该核心对 SDRC 研究人员的价值的基础。通过实施新技术，包括新颖的体外流动测定和多光子共焦活体显微镜，以及扩大对更传统的粘附和迁移测定技术的使用，核心将继续为用户提供尖端技术，以应用于他们的研究问题。此外，由 von Andrian 博士设计的在 T 细胞和/或特定 T 细胞亚群中表达荧光蛋白的小鼠已经生产出来并进行表征，现在可供 HSDRC 研究人员使用。白细胞迁移核心提供的服务可分为支持体外结合研究、体内归巢模型系统以及活体落射荧光和多光子显微镜技术。尽管存在相当大的重叠和合作，Fuhlbrigge 博士将继续为哈佛大学医学院的体外核心服务提供主要支持，而 von Andrian 博士将为血液研究中心的活体显微镜核心服务提供主要支持。这两个设施将继续支持整个动物体内归巢研究。